anti-IL6 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes IL6
  • 反应物种: Hu, Ms
  • 应用: ELISA, IHC-P, Neut, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: IL6
  • 抗原物种: Human
  • 抗原: E.coli derived Recombinant Human IL-6.(PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM)
  • Brand:
CAT.NO. : ARG56625
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes IL6
反应物种Hu, Ms
应用ELISA, IHC-P, Neut, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称IL6
抗原物种Human
抗原E.coli derived Recombinant Human IL-6.
(PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM)
偶联标记Un-conjugated
別名B-cell stimulatory factor 2; CDF; HSF; BSF-2; Interferon beta-2; IL-6; IFNB2; CTL differentiation factor; Interleukin-6; HGF; Hybridoma growth factor; BSF2; IFN-beta-2
应用说明
应用建议
应用推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56735 as a detection antibody
IHC-P1 µg/ml - 5 µg/ml
Neut0.20 - 0.40 µg/ml (To yield [ND50] of the biological activity of Human IL - 6 (1.0 ng/ml) )
WB0.1 - 1 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量25 kDa
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS (pH 7.2)
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 16193 Mouse IL6

GeneID: 3569 Human IL6

Swiss-port # P05231 Human Interleukin-6

Swiss-port # P08505 Mouse Interleukin-6

基因名称IL6
全名interleukin 6
背景介绍This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]
生物功能Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. [UniProt]
产品亮点Related products:
IL6 antibodies; IL6 ELISA Kits; IL6 recombinant proteins; Anti-Rabbit IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
预测分子量24 kDa
翻译后修饰N- and O-glycosylated.
检测图片 (4)
  • ARG56625 anti-IL6 antibody WB image

    Western blot: 50 µg of A549 untreated or treated with Herba C. The blots were stained with ARG56625 anti-IL6 antibody at 1:2000 dilution.

    Observed band MW: 25 kDa.

  • ARG56625 anti-IL6 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human cervical squamous cell carcinoma. The recommended ARG56625 anti-IL6 antibody concentration is 1 µg/ml - 5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56625 anti-IL6 antibody standard curve image

    Sandwich ELISA: ARG56625 anti-IL6 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56735 anti-IL6 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.

  • ARG56625 anti-IL6 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human cervical squamous cell carcinoma. The recommended ARG56625 anti-IL6 antibody concentration is 1 µg/ml - 5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

参考文献

Investigation Of Immunological Adjuvant Activities Of Panax Gienseng Saponins In Mouse Model

ELISA / Mouse

Khadija Gilani et al.
JPTCP.,  (2024)

publication_link

 

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Fecal microbiota transplantation inhibits colorectal cancer progression: Reversing intestinal microbial dysbiosis to enhance anti-cancer immune responses

WB / Mouse

Hao Yu et al.
Front Microbiol.,  (2023)

publication_link

 

hr_line

IFIT2-depleted metastatic oral squamous cell carcinoma cells induce muscle atrophy and cancer cachexia in mice

IHC-P / Mouse

Kuo-Chu Lai et al.
J Cachexia Sarcopenia Muscle.,  (2022)

publication_link

 

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