anti-Intra Acrosomal Protein antibody [Hs-14] (FITC)
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CAT.NO. : ARG53952
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Product Details
概述
| 产品描述 | FITC-conjugated Mouse Monoclonal antibody [Hs-14] recognizes Intra Acrosomal Protein |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS |
| 特异性 | The clone Hs-14 reacts with a 220 kDa testis-specific human intra-acrosomal protein associated with the membranes of the acrosomal vesicle._x000D_ |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | Hs-14 |
| 同位型 | IgM |
| 靶点名称 | Intra Acrosomal Protein |
| 抗原物种 | Human |
| 抗原 | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
| 偶联标记 | FITC |
| 別名 | D11S4365; Acrosomal vesicle protein 1; SP-10; Acrosomal protein SP-10; SPACA2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | TBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACRV1 |
| 全名 | acrosomal vesicle protein 1 |
| 背景介绍 | One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction. |
| 研究领域 | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody |
| 预测分子量 | 28 kDa |
检测图片 (1)
ARG53952 anti-Intra Acrosomal Protein antibody [Hs-14] (FITC) FACS image
Flow Cytometry: Separation of unstained (black) and stained (red) human sperm cells. Cells were stained with ARG53952 anti-Intra Acrosomal Protein antibody [Hs-14] (FITC) at 3 µg/ml dilution.
参考文献
克隆号文献
Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis.
[Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization].
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