anti-KIF5B / Kinesin Heavy Chain antibody [KN-02]
![anti-KIF5B / Kinesin Heavy Chain antibody [KN-02]](/upload/image/products/KN-02_1_210_205.jpg)
![anti-KIF5B / Kinesin Heavy Chain antibody [KN-02]](/upload/image/products/KN-02_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
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Brand:
CAT.NO. : ARG63049
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [KN-02] recognizes KIF5B / Kinesin Heavy Chain |
|---|---|
| 反应物种 | Hu, Ms, Rat, Pig |
| 应用 | ICC/IF |
| 特异性 | The clone KN-02 recognizes heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. KN-02 does not react with kinesin bound to taxol-stabilized microtubules. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | KN-02 |
| 同位型 | IgM |
| 靶点名称 | KIF5B / Kinesin Heavy Chain |
| 抗原物种 | Pig |
| 抗原 | Enriched fraction of porcine brain kinesin. |
| 偶联标记 | Un-conjugated |
| 別名 | HEL-S-61; Kinesin-1 heavy chain; Conventional kinesin heavy chain; KINH; KNS1; Ubiquitous kinesin heavy chain; UKHC; KNS |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by thiophilic adsorption-affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | TBS (pH 8.0) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KIF5B |
| 全名 | kinesin family member 5B |
| 背景介绍 | Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function. |
| 研究领域 | Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 110 kDa |
检测图片 (1)
ARG63049 anti-KIF5B / Kinesin Heavy Chain antibody [KN-02] ICC/IF image
Immunofluorescence: RBL-2H3 rat basophilic leukemia cells stained with ARG63049 anti-KIF5B / Kinesin Heavy Chain antibody [KN-02] (red). Nuclei stained with DAPI (blue).
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