anti-Ku 70 antibody

Key features and details

  • 产品描述: Mouse Monoclonal antibody recognizes Ku 70
  • 反应物种: Hu, Mk
  • 应用: ICC/IF, IP, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 同位型: IgG1
  • 靶点名称: Ku 70
  • 抗原物种: Human
  • 抗原: Purified recombinant human Ku70 protein fragments expressed in E.coli.
  • Brand:
CAT.NO. : ARG54158
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Product Details
概述
产品描述Mouse Monoclonal antibody recognizes Ku 70
反应物种Hu, Mk
应用ICC/IF, IP, WB
宿主Mouse
克隆Monoclonal
同位型IgG1
靶点名称Ku 70
抗原物种Human
抗原Purified recombinant human Ku70 protein fragments expressed in E.coli.
偶联标记Un-conjugated
別名DNA repair protein XRCC6; Thyroid-lupus autoantigen; Lupus Ku autoantigen protein p70; EC 4.2.99.-; EC 3.6.4.-; ATP-dependent DNA helicase II 70 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 6; CTC box-binding factor 75 kDa subunit; 70 kDa subunit of Ku antigen; CTC75; 5'-deoxyribose-5-phosphate lyase Ku70; KU70; TLAA; 5'-dRP lyase Ku70; CTCBF; ML8; G22P1; X-ray repair cross-complementing protein 6; ATP-dependent DNA helicase 2 subunit 1; Ku70
应用说明
应用建议
应用推荐稀释比
ICC/IF1:200
IPAssay-dependent
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量67 kDa
属性
形式Liquid
纯化Affinity purified
缓冲液PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 2547 Human XRCC6

Swiss-port # P12956 Human X-ray repair cross-complementing protein 6

基因名称XRCC6
全名X-ray repair complementing defective repair in Chinese hamster cells 6
背景介绍Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
生物功能Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. [UniProt]
细胞定位Nucleus. Chromosome
研究领域Cancer antibody; Gene Regulation antibody
预测分子量70 kDa
翻译后修饰Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
检测图片 (3)
  • ARG54158 anti-Ku 70 antibody ICC/IF image

    Immunofluorescence: HeLa cells fixed with -20°C Methanol and stained with ARG54158 anti-Ku 70 antibody at 1:200 dilution.

  • ARG54158 anti-Ku 70 antibody WB image

    Western blot: HeLa cell lysate stained with ARG54158 anti-Ku 70 antibody at 1:1000 dilution.

  • ARG54158 anti-Ku 70 antibody IP image

    Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54158 anti-Ku 70 antibody.

参考文献

Bis(chloroacetamidino)-Derived Heteroarene-Fused Anthraquinones Bind to and Cause Proteasomal Degradation of tNOX, Leading to c-Flip Downregulation and Apoptosis in Oral Cancer Cells

WB / Human

Jeng Shiun Chang et al.
Cancers (Basel).,  (2023)

publication_link

 

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Propolis alleviates 4-aminobiphenyl-induced oxidative DNA damage by inhibition of CYP2E1 expression in human liver cells

WB / Human

Eva Ari Wahyuni et al.
Environ Toxicol.,  (2021)

publication_link

 

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