anti-Lactoferrin antibody
CAT.NO. : ARG64744
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概述
产品描述 | Goat Polyclonal antibody recognizes Lactoferrin |
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反应物种 | Hu |
应用 | IHC-P, WB |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | Lactoferrin |
抗原物种 | Human |
抗原 | C-ENYKSQQSSDPDP |
偶联标记 | Un-conjugated |
別名 | LF; Lfcin-H; HEL110; Lactoferrin; Lactotransferrin; Growth-inhibiting protein 12; GIG12; Talalactoferrin; EC 3.4.21.-; HLF2 |
应用说明
应用建议 |
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应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
阳性对照 | Peripheral blood mononucleocyte | ||||||
实际分子量 | ~ 85 kDa |
属性
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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背景介绍 | This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody |
预测分子量 | 78 kDa |
翻译后修饰 | Isoform DeltaLf: Ubiquitinated at Lys-379 and Lys-391. Poly-N-acetyllactosaminic carbohydrate moiety seems to be needed for TLR4 activation. |
检测图片 (2)
ARG64744 anti-Lactoferrin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human blood vessel tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64744 anti-Lactoferrin antibody at 2 µg/ml dilution followed by AP-staining.
ARG64744 anti-Lactoferrin antibody WB image
Western blot: 35 µg of peripheral blood mononucleocyte lysate (in RIPA buffer) stained with ARG64744 anti-Lactoferrin antibody at 0.05 µg/ml dilution and incubated at RT for 1 hour.
