anti-Laminin 111 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes Laminin 111 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-Fr, WB |
| 特异性 | ARG10736 Laminin-111 antibody recognizes 3 laminin isotypes: α1 (440kDa), β1 (220kD) and γ1 (220kDa). |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Laminin 111 |
| 抗原物种 | Mouse |
| 抗原 | Laminin isolated from Mouse EHS cells. |
| 偶联标记 | Un-conjugated |
| 別名 | LAMA; PTBHS; S-LAM-alpha; Laminin subunit alpha-1; laminin, beta 1; laminin, gamma 1 |
| 应用建议 |
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| 应用说明 | Note: Based on the reference PMID: 35453580 This antibody may also suitable for IHC-P application in human samples. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification. |
| 缓冲液 | PBS and 50% Glycerol. |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | Lama1 |
| 全名 | laminin, alpha 1; laminin, beta 1; laminin, gamma 1 |
| 背景介绍 | This gene encodes one of the alpha 1 subunits of laminin. The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. These proteins make up a major component of the basement membrane and have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Mutations in this gene may be associated with Poretti-Boltshauser syndrome. [provided by RefSeq, Sep 2014] |
| 生物功能 | Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. [UniProt] |
| 细胞定位 | Secreted › extracellular space › extracellular matrix › basement membrane Note: Major component. |
| 预测分子量 | 337 kDa |
| 翻译后修饰 | Tyrosine phosphorylated by PKDCC/VLK. |
ARG10736 anti-Laminin 111 antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat brain stem stained with ARG10736 anti-Laminin 111 antibody (red) at 1:1000 dilution and costained with ARG52344 anti-Myelin Basic Protein antibody (green) at 1:5000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.)
The Laminin antibody is an excellent marker of basement membranes surrounding blood vessels, while the MBP antibody stains the myelin sheathes around axons.
ARG10736 anti-Laminin 111 antibody IHC-Fr image
Immunohistochemistry: Frozen sections of Mouse cortex stained with ARG10736 anti-Laminin 111 antibody (red). Blue is DAPI staining of DNA. This antibody reveals strong staining in the basement membranes of blood vessels.
ARG10736 anti-Laminin 111 antibody WB image
Western blot: 1) Rat heart cells lysates, and 2) 0.2 µg of purified laminin-111 protein from Mouse EHS sarcoma was stained with ARG10736 anti-Laminin 111 antibody. This antibody recognizes 3 laminin isotypes: α1 (440 kDa), β1 (220kD) and γ1 (220 kDa). Also recognized a laminin binding protein at 120 kDa in both Rat heart lysates and purified laminin protein. Since this protein always coexpresses with laminin this cross reactivity is irrelevant.
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