Anti-LC3B Rabbit Monoclonal Antibody [Clone RM293], 31-1177-00
![Anti-LC3B Rabbit Monoclonal Antibody [Clone RM293], 31-1177-00](/upload/image/products/Fig1_ICC_LC3B_e.png)
![Anti-LC3B Rabbit Monoclonal Antibody [Clone RM293], 31-1177-00](/upload/image/products/Fig2_WB_LC3B_e.png)
![Anti-LC3B Rabbit Monoclonal Antibody [Clone RM293], 31-1177-00](/upload/image/products/Fig3_LC3B_e.png)
Key features and details
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CAT.NO. : 31-1177-00
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Product Details
Product Details
| Product Name | Anti-LC3B Rabbit Monoclonal Antibody, Clone RM293 |
| Product Description | Rabbit monoclonal to LC3B |
| Catalog No. | 31-1177-00 |
| Clone Name | RM293 |
| Product Data Sheet | Anti-LC3B Rabbit Monoclonal Antibody Data Sheet RM293 |
| Specificity | RM293 reacts to human LC3B (Autophagy-related protein LC3 B) (Microtubule-associated proteins 1A/1B light chain 3B). It may also react to mouse or rat LC3B, as predicted by immunogen homology. |
| Immunogen | A peptide corresponding to the N-terminus of human LC3B. |
| Application | Immunohistochemistry (IHC) Immunocytochemistry (ICC) Western Blot (WB) |
| Species Reactivity | Human (Mouse, Rat) |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Immunohistochemistry (IHC): 1:100-1:200 dilution; Immunocytochemistry (ICC): 1:100-1:200 dilution; Western Blot (WB): 1:100-1:200 dilution. |
| Keywords | |
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Product Images
 | Immunocytochemical staining of HeLa cells untreated or treated with chloroquine (CHQ), using anti-LC3B rabbit monoclonal Antibody, Clone RM293 (red), at a 1:200 dilution. Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue). |
 | Western Blot of cell lysates of HeLa untreated or treated with chloroquine (CHQ), using anti-LC3B rabbit monoclonal antibody, Clone RM293, at a 1:200 dilution. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections using anti-LC3B rabbit monoclonal antibody, Clone RM293, at a 1:200 dilution. |
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