anti-LCAT / Lecithin Cholesterol Acyltransferase antibody
Key features and details
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CAT.NO. : ARG42192
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes LCAT / Lecithin Cholesterol Acyltransferase |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LCAT / Lecithin Cholesterol Acyltransferase |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 300-313 of Human LCAT / Lecithin Cholesterol Acyltransferase. (RDFQRFFADLHFEE) |
| 偶联标记 | Un-conjugated |
| 別名 | Phosphatidylcholine-sterol acyltransferase; Phospholipid-cholesterol acyltransferase; Lecithin-cholesterol acyltransferase; EC 2.3.1.43 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Rat brain, U87, HeLa and SMMC-7721 | ||||
| 实际分子量 | ~ 60 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P04180 Human Phosphatidylcholine-sterol acyltransferase |
|---|---|
| 基因名称 | LCAT |
| 全名 | lecithin-cholesterol acyltransferase |
| 背景介绍 | This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008] |
| 生物功能 | Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed:10329423, PubMed:19065001, PubMed:26195816). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines (PubMed:8820107). Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed:10722751). [UniProt] |
| 细胞定位 | Secreted. Note=Secreted into blood plasma (PubMed:3458198, PubMed:8820107, PubMed:10222237). Produced in astrocytes and secreted into cerebral spinal fluid (CSF). [UniProt] |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | O- and N-glycosylated. O-glycosylation on Thr-431 and Ser-433 consists of sialylated galactose beta 1-->3N-acetylgalactosamine structures. N-glycosylated sites contain sialylated triantennary and/or biantennary complex structures. [UniProt] |
检测图片 (1)
ARG42192 anti-LCAT / Lecithin Cholesterol Acyltransferase antibody WB image
Western blot: Rat brain, U87, HeLa and SMMC-7721 cell lysates stained with ARG42192 anti-LCAT / Lecithin Cholesterol Acyltransferase antibody.
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