anti-LEF1 antibody [SQab19160]
![anti-LEF1 antibody [SQab19160]](/upload/image/products/ARG66675_IHC-P_2_210_205.jpg)
![anti-LEF1 antibody [SQab19160]](/upload/image/products/ARG66675_IHC-P_1.jpg)
![anti-LEF1 antibody [SQab19160]](/upload/image/products/ARG66675_IHC-P_2.jpg)
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- 产品描述:
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- 克隆:
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- 靶点名称:
- 抗原物种:
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CAT.NO. : ARG66675
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Product Details
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab19160] recognizes LEF1 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab19160 |
| 同位型 | IgG |
| 靶点名称 | LEF1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 100-200 of Human LEF1. |
| 偶联标记 | Un-conjugated |
| 別名 | Lymphoid enhancer-binding factor 1; TCF1-alpha; TCF7L3; T cell-specific transcription factor 1-alpha; LEF-1; TCF1ALPHA; TCF10 |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q9UJU2 Human Lymphoid enhancer-binding factor 1 |
|---|---|
| 基因名称 | LEF1 |
| 全名 | lymphoid enhancer-binding factor 1 |
| 背景介绍 | This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
| 生物功能 | Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. [UniProt] |
| 细胞定位 | Nucleus. Note=Found in nuclear bodies upon PIASG binding. [UniProt] |
| 预测分子量 | 44 kDa |
| 翻译后修饰 | Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway. [UniProt] |
检测图片 (2)
ARG66675 anti-LEF1 antibody [SQab19160] IHC-P image
Immunohistochemistry: Paraffin-embedded Human Hodgkin's lymphoma tissue stained with ARG66675 anti-LEF1 antibody [SQab19160].
ARG66675 anti-LEF1 antibody [SQab19160] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human tonsil tissue stained with ARG66675 anti-LEF1 antibody [SQab19160]. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
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