anti-Leptin antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes Leptin
  • 反应物种: Ms
  • 应用: ELISA, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Leptin
  • 抗原物种: Mouse
  • 抗原: E.coli derived Recombinant Mouse Leptin.(MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC)
  • Brand:
CAT.NO. : ARG56701
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes Leptin
反应物种Ms
应用ELISA, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称Leptin
抗原物种Mouse
抗原E.coli derived Recombinant Mouse Leptin.
(MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC)
偶联标记Un-conjugated
別名Leptin; Obese protein; OB; LEPD; Obesity factor; OBS
应用说明
应用建议
应用推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56809 as a detection antibody
IHC-P0.20 µg/ml
WB0.1 - 0.2 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS (pH 7.2)
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 16846 Mouse LEP

Swiss-port # P41160 Mouse Leptin

基因名称Lep
全名leptin
背景介绍This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]
生物功能May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. [UniProt]
产品亮点Related products:
Leptin antibodies; Leptin ELISA Kits; Anti-Rabbit IgG secondary antibodies;
Related news:
A new role of fat – factory of circulating miRNAs
预测分子量19 kDa
检测图片 (3)
  • ARG56701 anti-Leptin antibody WB image

    Western blot: 250 - 0.24 ng of Mouse Leptin stained with ARG56701 anti-Leptin antibody, under reducing conditions.

  • ARG56701 anti-Leptin antibody WB image

    Western blot: 250 - 0.24 ng of Mouse Leptin stained with ARG56701 anti-Leptin antibody, under non-reducing conditions.

  • ARG56701 anti-Leptin antibody standard curve image

    Sandwich ELISA: ARG56701 anti-Leptin antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56809 anti-Leptin antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.

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