anti-LRTOMT antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes LRTOMT
  • 反应物种: Hu, Ms, Rat
  • 应用: IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: LRTOMT
  • 抗原物种: Human
  • 抗原: Synthetic peptide corresponding to a sequence of Human LRTOMT. (RLLTVERDPRTAAVAEKLIRLAGFDEHMVEL)
  • Brand:
CAT.NO. : ARG43132
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes LRTOMT
反应物种Hu, Ms, Rat
应用IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称LRTOMT
抗原物种Human
抗原Synthetic peptide corresponding to a sequence of Human LRTOMT. (RLLTVERDPRTAAVAEKLIRLAGFDEHMVEL)
偶联标记Un-conjugated
別名Protein LRTOMT1; LRRC51; CFAP111; DFNB63; Leucine-rich repeat-containing protein 51
应用说明
应用建议
应用推荐稀释比
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
应用说明IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量~ 27 kDa
属性
形式Liquid
纯化Immunogen affinity purified.
缓冲液0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
抗菌剂0.05% Sodium azide
稳定剂4% Trehalose
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 220074 Human LRTOMT

GeneID: 308868 Rat LRTOMT

Swiss-port # B6CZ62 Rat Transmembrane O-methyltransferase

Swiss-port # Q96E66 Human Leucine-rich repeat-containing protein 51

基因名称LRTOMT
全名leucine rich transmembrane and O-methyltransferase domain containing
背景介绍This gene has evolved in primates as a fusion of two ancestral neighboring genes, Lrrc51 and Tomt, which exist as two independent genes in rodents. The fusion gene contains some shared exons, but encodes structurally unrelated proteins, LRTOMT1 and LRTOMT2. Those variants that utilize the more centromere-proximal 3' terminal exon (short transcript form) encode LRTOMT1, while those variants that use a more centromere-distal 3' terminal exon (long transcript form) encode the LRTOMT2 protein. There is a small region within one of the exons of this gene that contains overlapping alternate reading frames for both LRTOMT1 and LRTOMT2. LRTOMT1 shares similarity with the protein encoded by mouse Lrrc51, while LRTOMT2 shares similarity with the protein encoded by mouse Tomt. Alternative splicing results in multiple transcript variants, encoding different isoforms of both LRTOMT1 and LRTOMT2. The LRTOMT1 protein is a leucine-rich repeat-containing protein, while the LRTOMT2 protein is a catechol-O-methyltransferase that catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to a hydroxyl group of catechols and is essential for auditory and vestibular function. Mutations in this gene have been associated with nonsyndromic deafness. [provided by RefSeq, Nov 2017]
细胞定位Cytoplasm. [UniProt]
预测分子量22 kDa
检测图片 (7)
  • ARG43132 anti-LRTOMT antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43132 anti-LRTOMT antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG43132 anti-LRTOMT antibody WB image

    Western blot: 50 µg of sample under reducing conditions. Human placenta, MCF-7, HeLa, Caco-2, K562, U2OS and THP-1 whole cell lysates stained with ARG43132 anti-LRTOMT antibody at 0.5 µg/ml dilution, overnight at 4°C.

  • ARG43132 anti-LRTOMT antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43132 anti-LRTOMT antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG43132 anti-LRTOMT antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43132 anti-LRTOMT antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG43132 anti-LRTOMT antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43132 anti-LRTOMT antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG43132 anti-LRTOMT antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43132 anti-LRTOMT antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG43132 anti-LRTOMT antibody WB image

    Western blot: 50 µg of sample under reducing conditions. Rat brain, Rat ovary, Rat heart, Rat lung, Mouse brain and Mouse lung lysates stained with ARG43132 anti-LRTOMT antibody at 0.5 µg/ml dilution, overnight at 4°C.

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