anti-LXR alpha + LXR beta antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
-
Brand:
CAT.NO. : ARG63670
US＄ Please choose
US＄ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。
Product Details
概述
| 产品描述 | Goat Polyclonal antibody recognizes LXR alpha + LXR beta |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog |
| 应用 | FACS, ICC/IF, WB |
| 特异性 | This antibody is expected to recognise an epitope corresponding to aa 429-442 of human LXR alpha protein (NP_005684.2) and to aa 443-456 of human LXR beta protein (NP_009052.3). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | LXR alpha + LXR beta |
| 抗原物种 | Pig |
| 抗原 | CRLQDKKLPPLLSEI |
| 偶联标记 | Un-conjugated |
| 別名 | LXR alpha: RLD-1; Oxysterols receptor LXR-alpha; Nuclear receptor subfamily 1 group H member 3; LXRA; LXR-a; Liver X receptor alpha LXR beta: Ubiquitously-expressed nuclear receptor; UNR; Liver X receptor beta; NER; RIP15; Oxysterols receptor LXR-beta; LXRB; Nuclear receptor subfamily 1 group H member 2; Nuclear receptor NER; LXR-b; NER-I |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | NR1H3; NR1H2 |
|---|---|
| 全名 | nuclear receptor subfamily 1, group H, member 3; nuclear receptor subfamily 1, group H, member 2 |
| 背景介绍 | The liver X receptors, LXRA (NR1H3; MIM 602423) and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al., 2009 [PubMed 19436111]).[supplied by OMIM, Jan 2010] |
| 生物功能 | LXR alpha: Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed:19481530, PubMed:25661920). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES (By similarity). LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8 (PubMed:19481530). Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). [UniProt] LXR beta: Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed:25661920). Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8; DLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (PubMed:20159957). |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody |
| 预测分子量 | LXR alpha: 50 kDa LXR beta: 51 kDa |
检测图片 (3)
ARG63670 anti-LXR alpha + LXR beta antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A549 cells permeabilized with 0.15% Triton. Cells were stained with ARG63670 anti-LXR alpha + LXR beta antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
ARG63670 anti-LXR alpha + LXR beta antibody WB image
Western blot: 35 µg of Human liver (A) and Human kidney (B) lysates (in RIPA buffer) stained with ARG63670 anti-LXR alpha + LXR beta antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
ARG63670 anti-LXR alpha + LXR beta antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed A549 cells permeabilized with 0.5% Triton. Cells were stained with ARG63670 anti-LXR alpha + LXR beta antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
New Products
