anti-M13 Bacteriophage g3p coat protein antibody [E1]
CAT.NO. : ARG23605
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概述
| 产品描述 | Mouse Monoclonal antibody [E1] recognizes M13 Bacteriophage g3p coat protein. This product recognizes the gp3 (G3P) phage coat protein of the M13 bacteriophage widely used in the preparation of "phage-display" immunoglobulin libraries. G3P plays a role in the entry of the viral genome into the bacterial host. This antibody may be used to detect binding of ScFv antibodies developed in the M13 system. |
|---|---|
| 反应物种 | Virus |
| 应用 | ELISA, FACS, IHC-Fr |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | E1 |
| 同位型 | IgG2a |
| 靶点名称 | M13 Bacteriophage g3p coat protein |
| 抗原物种 | Virus |
| 抗原 | M13 Phage coat proteins. |
| 偶联标记 | Un-conjugated |
| 別名 | Coat protein A; g3p; Minor coat protein; pIII; M13 antibody (g3p); Attachment protein G3P |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS and 0.09% Sodium azide. |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
检测图片 (1)
ARG23605 anti-M13 Bacteriophage g3p coat protein antibody [E1] FACS image
Flow Cytometry: Colo320 cell line stained with a cell-line specific M13 phage antibody (filled profile), compared to an irrelevant phage antibody (open profile). Cells were incubated overnight with 1012 phage particles, then detected with 10 µg/ml ARG23605 anti-M13 Bacteriophage g3p coat protein antibody [E1] followed by Rabbit anti-Mouse IgG (FITC).
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