anti-M13 Bacteriophage g8p coat protein antibody [SQab21250]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [SQab21250] recognizes M13 Bacteriophage g8p coat protein
  • 反应物种: Virus
  • 应用: ELISA, FACS
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: SQab21250
  • 同位型: IgG2b
  • 靶点名称: M13 Bacteriophage g8p coat protein
  • 抗原物种: Virus
  • Brand:
CAT.NO. : ARG66892
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Product Details
概述
产品描述Mouse Monoclonal antibody [SQab21250] recognizes M13 Bacteriophage g8p coat protein
反应物种Virus
应用ELISA, FACS
宿主Mouse
克隆Monoclonal
克隆号SQab21250
同位型IgG2b
靶点名称M13 Bacteriophage g8p coat protein
抗原物种Virus
抗原g8p coat protein combine M13KO7 phage.
偶联标记Un-conjugated
別名M13 Bacteriophage coat phage protein pVIII; M13 pVIII protein; M13 pVIII coat protein; M13 antibody (g8p); Anti-M13 Bacteriophage g8p Coat Protein Antibody, SQab21250, Arigo Biolaboratories, Western blot, ELISA, immunoprecipitation, M13 bacteriophage, DNA cloning, sequencing, g8p coat protein, molecular biology applications, research use
应用说明
应用建议
应用推荐稀释比
ELISA1:5000 - 1:75000
FACS1:500 - 1:1000
应用说明This antibody reacts phage by ELISA and flow cytometry, but does not reacts to recombinant Fc-tagged g8p protein.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
纯度95% (by SDS-PAGE)
缓冲液PBS and 0.01% Sodium azide.
抗菌剂0.01% Sodium azide
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
基因名称VIII (M13)
全名Capsid protein G8P (M13)
生物功能M13 Bacteriophage g8p coat protein self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane. [UniProtKB - P69541 (CAPSD_BPM13)]
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检测图片 (4)
  • ARG66892 anti-M13 Bacteriophage g8p coat protein antibody [SQab21250] ELISA image

    ELISA: M13 phage was pre-coated on the plate and detected the phage by variable concentrations of ARG66892 anti-M13 Bacteriophage g8p coat protein antibody [SQab21250]. Isotype control: Normal mouse IgG.

  • ARG66892 anti-M13 Bacteriophage g8p coat protein antibody [SQab21250] FACS image

    Flow Cytometry: MCF7 cells incubated with 1x10^9 (blue) or 1x10^10 (red) of anti-HER3 scFvs displayed phage, then stained with ARG66892 anti-M13 Bacteriophage g8p coat protein antibody [SQab21250] at 1:500 dilution. And a PE-labeled anti-mouse IgG antibody used as the third antibody. Cells did not incubate the anti-HER3 scFvs displayed phage as control sample (black).

  • ARG66892 anti-M13 Bacteriophage g8p coat protein antibody [SQab21250] ELISA image

    ELISA: The plate was coated with 10^8 of M13 bacteriophages. Samples were detected with serially diluted ARG66892 anti-M13 Bacteriophage g8p coat protein antibody [SQab21250].

  • ARG66892 anti-M13 Bacteriophage g8p coat protein antibody [SQab21250] ELISA image

    Sensitivity analysis: Coating the indicated amounts of M13 bacteriophages and stained with the different concentration of ARG66892 anti-M13 Bacteriophage g8p coat protein antibody [SQab21250].

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