anti-MARCKS antibody
Key features and details
- 产品描述:
- 反应物种:
- 不反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG11133
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MARCKS |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MARCKS |
| 抗原物种 | Human |
| 抗原 | Full-length recombinant Human MARCKS. |
| 偶联标记 | Un-conjugated |
| 別名 | MACS; 80K-L; Myristoylated alanine-rich C-kinase substrate; PKCSL; Protein kinase C substrate, 80 kDa protein, light chain; 80K-L protein; MARCKS; PRKCSL |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HEK293, HeLa and SH-SY5Y | ||||||
| 实际分子量 | ~ 80 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS, 5 mM Sodium azide and 50% Glycerol. |
| 抗菌剂 | 5 mM Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P29966 Human Myristoylated alanine-rich C-kinase substrate |
|---|---|
| 基因名称 | MARCKS |
| 全名 | myristoylated alanine-rich protein kinase C substrate |
| 背景介绍 | The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008] |
| 生物功能 | MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton. Membrane; Lipid-anchor. [UniProt] |
| 预测分子量 | 32 kDa |
| 翻译后修饰 | Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. [UniProt] |
检测图片 (2)
ARG11133 anti-MARCKS antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG11133 anti-MARCKS antibody (red) at 1:1000 dilution, and co-stained with anti-beta Tubulin antibody (green) at 1:10000 dilution. DAPI (blue) for nuclear staining.
The MARCKS antibody recognizes protein localized in the plasma membrane and cytoplasm, while the beta Tubulin antibody stains the network of cytoplasmic microtubules.
ARG11133 anti-MARCKS antibody WB image
Western blot: HEK293, HeLa, SH-SY5Y, COS1, NIH/3T3 and C6 cell lysates stained with ARG11133 anti-MARCKS antibody (green) at 1:1000 dilution and ARG52320 anti-GAPDH antibody [1D4] (red) at 1:5000 dilution.
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