anti-MBNL1 antibody [1H1]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [1H1] recognizes MBNL1
  • 反应物种: Hu
  • 预测物种: Chk
  • 不反应物种: Ms, Rat
  • 应用: ICC/IF, IHC-Fr, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 1H1
  • 同位型: IgG1
  • Brand:
CAT.NO. : ARG10721
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Product Details
概述
产品描述Mouse Monoclonal antibody [1H1] recognizes MBNL1
反应物种Hu
预测物种Chk
不反应物种Ms, Rat
应用ICC/IF, IHC-Fr, WB
宿主Mouse
克隆Monoclonal
克隆号1H1
同位型IgG1
靶点名称MBNL1
抗原物种Human
抗原Full-length recombinant Human MBNL1.
偶联标记Un-conjugated
別名Muscleblind-like protein 1; Triplet-expansion RNA-binding protein; MBNL; EXP
应用说明
应用建议
应用推荐稀释比
ICC/IF1:1000
IHC-Fr1:1000
WB1:1000 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification.
缓冲液PBS and 50% Glycerol.
稳定剂50% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 4154 Human MBNL1

Swiss-port # Q9NR56 Human Muscleblind-like protein 1

基因名称MBNL1
全名muscleblind-like splicing regulator 1
背景介绍This gene encodes a member of the muscleblind protein family which was initially described in Drosophila melanogaster. The encoded protein is a C3H-type zinc finger protein that modulates alternative splicing of pre-mRNAs. Muscleblind proteins bind specifically to expanded dsCUG RNA but not to normal size CUG repeats and may thereby play a role in the pathophysiology of myotonic dystrophy. Mice lacking this gene exhibited muscle abnormalities and cataracts. Several alternatively spliced transcript variants have been described but the full-length natures of only some have been determined. The different isoforms are thought to have different binding specificities and/or splicing activities. [provided by RefSeq, Sep 2015]
生物功能Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues. [UniProt]
预测分子量42 kDa
检测图片 (4)
  • ARG10721 anti-MBNL1 antibody [1H1] ICC/IF image

    Immunocytochemistry: HeLa cells were stained with ARG10721 anti-MBNL1 antibody [1H1] at 1: 1000. Muscleblind-like protein 1 is present in the nucleus of HeLa cells (red). Co-staining is with a rabbit polyclonal antibody against vimentin (red) reveals cytoplasmic intermediate filaments.  Blue is a DNA stain which largely colocalizes with the MBNL1 protein in the nucleus.

  • ARG10721 anti-MBNL1 antibody [1H1] ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG10721 anti-MBNL1 antibody [1H1] (green) at 1:1000 dilution and costained with ARG10757 anti-Hsp 60 antibody (red) at 1:5000 dilution.

    The MBNL1 antibody specifically labels nuclei, while the Hsp 60 antibody reveals protein expressed in mitochondria.

  • ARG10721 anti-MBNL1 antibody [1H1] WB image

    Western blot: HL60 cell lysate was stained with ARG10721 anti-MBNL1 antibody [1H1] at 1:1000 dilution.

  • ARG10721 anti-MBNL1 antibody [1H1] WB image

    Western blot: HEK293 (cytosol), HEK293 (nuclear), NIH/3T3 (cytosol), NIH/3T3 (nuclear), HeLa (cytosol) and HeLa (nuclear). The blots were stained with ARG10721 anti-MBNL1 antibody [1H1] (green) at 1:1000 dilution.

    MBNL1 protein is predominantly detected in the nuclear enriched fractions. This antibody binds to the Human protein and does not bind the rodent homologue.

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