anti-mCherry antibody [1C51]

CAT.NO. : ARG10759

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Size:

50 μl

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal Antibody to mCherry
反应物种	Other
应用	ICC/IF, IHC-Fr, WB
宿主	Mouse
克隆	Monoclonal
克隆号	1C51
同位型	IgG2a
靶点名称	mCherry
抗原物种	Others
抗原	Full length recombinant protein.
偶联标记	Un-conjugated

应用说明

应用建议

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purification.
缓冲液	PBS and 50% Glycerol.
稳定剂	50% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

检测图片 (3)

ARG10759 anti-mCherry antibody [1C51] ICC/IF image
Immunocytochemistry: HEK293 cells transfected with mCherry and visualized in red. The cells were stained with ARG10759 anti-mCherry antibody [1C51] in green, and visualized using a confocal microscope. Transfected cells are yellow, showing overlap of the mCherry and the ARG10759 anti-mCherry antibody [1C51]. Untransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue).
ARG10759 anti-mCherry antibody [1C51] WB image
Western blot: Crude extract of HEK293 cells transfected with pFin-EF1-mCherry vector in lane labelled "+". The "-" lane is a blot of an equal amount of protein extract from untransfected HEK293 cells. ARG10759 anti-mCherry antibody [1C51] binds a major band running at ~28 kDa corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected HEK293 cells.
ARG10759 anti-mCherry antibody [1C51] WB image
Western blot: 1) HEK293 (non-transfected), 2) HEK293 transfected with mCherry-HA construct, 3) Recombinant mCherry protein, 4) Recombinant GFP protein and 5) HEK293 transfected with GFP construct. The blots were stained with ARG10759 anti-mCherry antibody [1C51] (green) at 1:1000 dilution. The same blot was simultaneously stained with ARG10757 anti-Hsp 60 antibody (red) at 1:5000 dilution.

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