anti-MCL1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG55381
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MCL1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MCL1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 191-226 of Human MCL1. |
| 偶联标记 | Un-conjugated |
| 別名 | Bcl-2-related protein EAT/mcl1; BCL2L3; MCL1S; Induced myeloid leukemia cell differentiation protein Mcl-1; MCL1-ES; mcl1/EAT; Bcl-2-like protein 3; EAT; Mcl-1; bcl2-L-3; Bcl2-L-3; MCL1L; TM |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MCL1 |
| 全名 | myeloid cell leukemia 1 |
| 背景介绍 | This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010] |
| 生物功能 | Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. [UniProt] |
| 细胞定位 | Membrane; Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Note=Cytoplasmic, associated with mitochondria |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody |
| 预测分子量 | 37 kDa |
| 翻译后修饰 | Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment. Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region. Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites. Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159. |
检测图片 (4)
ARG55381 anti-MCL1 antibody WB image
Western blot: 30 µg of 293T and HCT116 cell lysates stained with ARG55381 anti-MCL1 antibody at 1:500 dilution.
ARG55381 anti-MCL1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma stained with ARG55381 anti-MCL1 antibody.
ARG55381 anti-MCL1 antibody WB image
Western blot: 20 µg of Rat heart lysate stained with ARG55381 anti-MCL1 antibody at 1:2000 dilution.
ARG55381 anti-MCL1 antibody FACS image
Flow Cytometry: ZR-75-1 cells stained with ARG55381 anti-MCL1 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
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