anti-MDH2 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes MDH2
  • 反应物种: Hu, Ms
  • 预测物种: Bov, Rat, Mk, Pig, Yeast
  • 应用: FACS, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: MDH2
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG55688
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes MDH2
反应物种Hu, Ms
预测物种Bov, Rat, Mk, Pig, Yeast
应用FACS, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称MDH2
抗原物种Human
抗原KLH-conjugated synthetic peptide corresponding to aa. 86-115 (Center) of Human MDH2.
偶联标记Un-conjugated
別名MDH; MOR1; MGC:3559; Malate dehydrogenase, mitochondrial; EC 1.1.1.37; M-MDH
应用说明
应用建议
应用推荐稀释比
FACS1:10 - 1:50
IHC-P1:10 - 1:50
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照HeLa
属性
形式Liquid
纯化This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
缓冲液PBS and 0.09% (W/V) Sodium azide.
抗菌剂0.09% (W/V) Sodium azide.
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 17448 Mouse MDH2

GeneID: 4191 Human MDH2

Swiss-port # P08249 Mouse Malate dehydrogenase, mitochondrial

Swiss-port # P40926 Human Malate dehydrogenase, mitochondrial

基因名称MDH2
全名malate dehydrogenase 2, NAD (mitochondrial)
背景介绍Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
细胞定位Mitochondrion matrix.
预测分子量36 kDa
翻译后修饰Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri- and tetraacetylations. Glucose also increases acetylation by about 60%.
检测图片 (3)
  • ARG55688 anti-MDH2 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma stained with ARG55688 anti-MDH2 antibody.

  • ARG55688 anti-MDH2 antibody WB image

    Western blot: 35 µg of HeLa cell lysate stained with ARG55688 anti-MDH2 antibody.

  • ARG55688 anti-MDH2 antibody FACS image

    Flow Cytometry: K562 cells stained with ARG55688 anti-MDH2 antibody (bottom histogram) or without primary antibody as control (top histogram), followed by incubation with FITC labelled secondary antibody.

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