anti-MED15 / PCQAP antibody [6F4]

CAT.NO. : ARG43231

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Size:

50 μg

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [6F4] recognizes MED15 / PCQAP
反应物种	Hu
应用	FACS, ICC/IF, WB
宿主	Mouse
克隆	Monoclonal
克隆号	6F4
同位型	IgG1
靶点名称	MED15 / PCQAP
抗原物种	Human
抗原	Recombinant protein corresponding to M1-A285 of Human MED15 / PCQAP.
偶联标记	Un-conjugated
別名	Activator-recruited cofactor 105 kDa component; PC2 glutamine/Q-rich-associated protein; Mediator complex subunit 15; TPA-inducible gene 1 protein; ARC105; Positive cofactor 2 glutamine/Q-rich-associated protein; CTG7A; Trinucleotide repeat-containing gene 7 protein; Mediator of RNA polymerase II transcription subunit 15; CTG repeat protein 7a; CAG7A; TIG-1; TIG1; PCQAP; TNRC7

应用说明

应用建议

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

实际分子量

~ 87 kDa

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
抗菌剂	0.05% Sodium azide
稳定剂	4% Trehalose
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 51586 Human MED15 Swiss-port # Q96RN5 Human Mediator of RNA polymerase II transcription subunit 15
基因名称	MED15
全名	mediator complex subunit 15
背景介绍	The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
生物功能	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway. [UniProt]
细胞定位	Cytoplasm. Nucleus. [UniProt]
预测分子量	87 kDa
翻译后修饰	Ubiquitinated by TRIM11, leading to proteasomal degradation. [UniProt]

检测图片 (3)

ARG43231 anti-MED15 / PCQAP antibody [6F4] ICC/IF image
Immunofluorescence: A431 cells stained with ARG43231 anti-MED15 / PCQAP antibody [6F4] (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
ARG43231 anti-MED15 / PCQAP antibody [6F4] WB image
Western blot: 50 µg of sample under reducing conditions. K562, U87, Caco-2, HL-60, HepG2, HeLa and PC-3 whole cell lysates stained with ARG43231 anti-MED15 / PCQAP antibody [6F4] at 0.5 µg/ml dilution, overnight at 4°C.
ARG43231 anti-MED15 / PCQAP antibody [6F4] FACS image
Flow Cytometry: U2OS cells were blocked with 10% normal goat serum and then stained with ARG43231 anti-MED15 / PCQAP antibody [6F4] (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was mouse IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

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