anti-MEK1 phospho (Thr386) antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes MEK1 phospho (Thr386)
  • 反应物种: Hu, Rat
  • 预测物种: Ms, Bov, Chk, Dog, NHuPrm, Xenopus laevis
  • 应用: WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: MEK1
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG52335
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes MEK1 phospho (Thr386)
反应物种Hu, Rat
预测物种Ms, Bov, Chk, Dog, NHuPrm, Xenopus laevis
应用WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称MEK1
抗原物种Human
抗原Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr386 conjugated to KLH
偶联标记Un-conjugated
別名MEK 1; PRKMK1; MAPKK 1; EC 2.7.12.2; MEK1; MAPKK1; MKK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPK/ERK kinase 1; CFC3; ERK activator kinase 1
应用说明
应用建议
应用推荐稀释比
WB1:1000
应用说明Specific for the ~45k MEK 1 protein phosphorylated at Thr386. The immunolabeling is completely eliminated by treatment with λ-phosphatase.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity Purified
缓冲液10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
稳定剂0.1 mg/ml BSA, 50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 170851 Rat MAP2K1

GeneID: 5604 Human MAP2K1

Swiss-port # Q01986 Rat Dual specificity mitogen-activated protein kinase kinase 1

Swiss-port # Q02750 Human Dual specificity mitogen-activated protein kinase kinase 1

基因名称MAP2K1
全名mitogen-activated protein kinase kinase 1
背景介绍MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).
研究领域Signaling Transduction antibody
预测分子量43 kDa
翻译后修饰Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (RAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK.
Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
检测图片 (1)
  • ARG52335 anti-MEK1 phospho (Thr386) antibody WB image

    Western blot: Human T47D cells showing phospho-specific immunolabeling of the ~45 kDa MEK1 protein phosphorylated at Thr386 stained with ARG52335 anti-MEK1 phospho (Thr386) antibody.

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