anti-MEK3 + MEK6 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG41989
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MEK3 + MEK6 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MEK3 + MEK6 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human MEK3 / MEK6. |
| 偶联标记 | Un-conjugated |
| 別名 | MEK3: SAPK kinase 2; MEK 3; MAPKK 3; Stress-activated protein kinase kinase 2; PRKMK3; EC 2.7.12.2; MAPK/ERK kinase 3; MAPKK3; SAPKK2; SAPKK-2; MAP kinase kinase 3; MKK3; Dual specificity mitogen-activated protein kinase kinase 3 MEK6: SAPK kinase 3; MEK 6; MAPKK 6; MAPK/ERK kinase 6; EC 2.7.12.2; PRKMK6; SAPKK3; MAPKK6; SAPKK-3; Stress-activated protein kinase kinase 3; MKK6; MAP kinase kinase 6; Dual specificity mitogen-activated protein kinase kinase 6 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| 阳性对照 | HeLa | ||||||||||||
| 实际分子量 | 37 and 39 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | MAP2K3; MAP2K6 |
|---|---|
| 全名 | mitogen-activated protein kinase kinase 3 mitogen-activated protein kinase kinase 6 |
| 背景介绍 | MEK3: The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008] MEK6: This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq, Jul 2008] |
| 生物功能 | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases. [UniProt] |
| 细胞定位 | MEK6: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton. Note=Binds to microtubules. [UniProt] |
| 预测分子量 | MEK3: 39 kDa MEK6: 37 kDa |
| 翻译后修饰 | MEK3: Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity (PubMed:8622669). Phosphorylated by TAOK2 (PubMed:11279118). Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. [UniProt] MEK6: Weakly autophosphorylated. Phosphorylated at Ser-207 and Thr-211 by the majority of M3Ks, such as MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK3, MAP3K4/MEKK4, MAP3K7/TAK1, MAP3K11/MLK3 and MAP3K17/TAOK2. Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. [UniProt] |
检测图片 (3)
ARG41989 anti-MEK3 + MEK6 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG41989 anti-MEK3 + MEK6 antibody.
ARG41989 anti-MEK3 + MEK6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spleen tissue stained with ARG41989 anti-MEK3 + MEK6 antibody.
ARG41989 anti-MEK3 + MEK6 antibody WB image
Western blot: HeLa cell lysate stained with ARG41989 anti-MEK3 + MEK6 antibody.
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