anti-METTL16 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG43405
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes METTL16 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | METTL16 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 263-562 of Human METTL16 (NP_076991.3). |
| 偶联标记 | Un-conjugated |
| 別名 | Methyltransferase 10 domain-containing protein; METT10D; Methyltransferase-like protein 16; EC 2.1.1.- |
应用说明
| 应用建议 |
| ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | A549 | ||||||||||
| 实际分子量 | ~ 74 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | METTL16 |
| 全名 | methyltransferase like 16 |
| 生物功能 | RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753, PubMed:30197299, PubMed:30197297). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753, PubMed:30197299, PubMed:30197297). Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing and/or stability of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression (PubMed:28525753). In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753, PubMed:29051200). Also able to bind various lncRNAs (PubMed:29051200). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311). [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 64 kDa |
检测图片 (4)
ARG43405 anti-METTL16 antibody ICC/IF image
Immunofluorescence: C6 cells stained with ARG43405 anti-METTL16 antibody at 1:100 dilution.
ARG43405 anti-METTL16 antibody WB image
Western blot: 25 µg of A549 cell lysate stained with ARG43405 anti-METTL16 antibody at 1:1000 dilution.
ARG43405 anti-METTL16 antibody IP image
Immunoprecipitation: 300 µg extracts of LNCaP cells were immunoprecipitated and stained with ARG43405 anti-METTL16 antibody at 1:1000 dilution.
ARG43405 anti-METTL16 antibody WB image
Western blot: 25 µg of Rat testis lysate stained with ARG43405 anti-METTL16 antibody at 1:1000 dilution.
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