anti-MHC Class II antibody [M5/114] (FITC)

产品描述: FITC-conjugated Rat Monoclonal antibody [M5/114] recognizes MHC Class II
反应物种: Ms
应用: FACS
特异性: The clone M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
宿主: Rat
克隆: Monoclonal
克隆号: M5/114
同位型: IgG2b
靶点名称: MHC Class II

CAT.NO. : ARG63071

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Size:

100 μg

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	FITC-conjugated Rat Monoclonal antibody [M5/114] recognizes MHC Class II
反应物种	Ms
应用	FACS
特异性	The clone M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
宿主	Rat
克隆	Monoclonal
克隆号	M5/114
同位型	IgG2b
靶点名称	MHC Class II
抗原物种	Mouse
抗原	Activated C57BL/6 mouse spleen cells.
偶联标记	FITC
別名	HLA-DRB; HLA class II histocompatibility antigen, DRB1-3 chain; SS1; MHC class II antigen DRB1*3; HLA-DR1B; DRw10; Clone P2-beta-3; DRB1

应用说明

应用建议

应用	推荐稀释比
FACS	2 - 3 µg/ml

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
缓冲液	PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	0.5 mg/ml
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称	H2-D1
全名	histocompatibility 2, D region locus 1
背景介绍	MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
生物功能	Involved in the presentation of foreign antigens to the immune system. [UniProt]
研究领域	Immune System antibody
预测分子量	30 kDa
翻译后修饰	Ubiquitinated by MARCH1 and MARCH8 at Lys-254 leading to sorting into the endosome system and down-regulation of MHC class II.

检测图片 (1)

ARG63071 anti-MHC Class II antibody [M5/114] (FITC) FACS image
Flow Cytometry: Mouse splenocytes stained with ARG63071 anti-MHC Class II antibody [M5/114] (FITC).

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