anti-MID1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG40448
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MID1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MID1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 478-667 of Human MID1 (NP_000372.1). |
| 偶联标记 | Un-conjugated |
| 別名 | OSX; MIDIN; RING finger protein Midline-1; FXY; EC 6.3.2.-; E3 ubiquitin-protein ligase Midline-1; RNF59; Midin; TRIM18; Tripartite motif-containing protein 18; XPRF; ZNFXY; RING finger protein 59; GBBB1; OGS1; BBBG1; Putative transcription factor XPRF; OS |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse heart | ||||||
| 实际分子量 | 85 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # O15344 Human E3 ubiquitin-protein ligase Midline-1 Swiss-port # O70583 Mouse E3 ubiquitin-protein ligase Midline-1 |
|---|---|
| 基因名称 | MID1 |
| 全名 | midline 1 |
| 背景介绍 | The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Multiple different transcript variants are generated by alternate splicing; however, the full-length nature of some of the variants has not been determined. [provided by RefSeq, Jul 2010] |
| 生物功能 | Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination. [UniProt] |
| 细胞定位 | Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Note=Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis. [UniProt] |
| 预测分子量 | 75 kDa |
| 翻译后修饰 | Phosphorylated on serine and threonine residues. [UniProt] |
检测图片 (2)
ARG40448 anti-MID1 antibody ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG40448 anti-MID1 antibody.
ARG40448 anti-MID1 antibody WB image
Western blot: 25 µg of Mouse heart lysate stained with ARG40448 anti-MID1 antibody at 1:1000 dilution.
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