anti-MIF antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes MIF
  • 反应物种: Hu
  • 预测物种: Ms, Rat
  • 应用: IHC-P, WB
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: MIF
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG63789
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes MIF
反应物种Hu
预测物种Ms, Rat
应用IHC-P, WB
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称MIF
抗原物种Human
抗原C-NAANVGWNNSTFA
偶联标记Un-conjugated
別名Phenylpyruvate tautomerase; L-dopachrome tautomerase; GLIF; L-dopachrome isomerase; MMIF; Macrophage migration inhibitory factor; MIF; GIF; EC 5.3.2.1; Glycosylation-inhibiting factor; EC 5.3.3.12
应用说明
应用建议
应用推荐稀释比
IHC-P5 µg/ml
WB0.01 - 0.03 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 4282 Human MIF

Swiss-port # P14174 Human Macrophage migration inhibitory factor

背景介绍This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
研究领域Immune System antibody
预测分子量12 kDa
检测图片 (3)
  • ARG63789 anti-MIF antibody WB image

    Western Blot: Daudi (A) and Jurkat (B) lysate (35 µg protein in RIPA buffer) stained with ARG63789 anti-MIF antibody at 0.01 µg/ml dilution.

  • ARG63789 anti-MIF antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human thymus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63789 anti-MIF antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG63789 anti-MIF antibody WB image

    Western blot: 35 µg of Human thymus lysate (in RIPA buffer) stained with ARG63789 anti-MIF antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.

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