Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM140]; H3K4me1; Histone H3 (monomethyl K4); 31-1046-00; 31-1046-00-S
![Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM140]; H3K4me1; Histone H3 (monomethyl K4); 31-1046-00; 31-1046-00-S](/upload/image/products/WB2.png)
![Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM140]; H3K4me1; Histone H3 (monomethyl K4); 31-1046-00; 31-1046-00-S](/upload/image/products/rm140_1.png)
![Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM140]; H3K4me1; Histone H3 (monomethyl K4); 31-1046-00; 31-1046-00-S](/upload/image/products/RM140-Fig3_e.png)
![Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM140]; H3K4me1; Histone H3 (monomethyl K4); 31-1046-00; 31-1046-00-S](/upload/image/products/Fig4_RM140_ICC.png)
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CAT.NO. : 31-1046-00
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Product Details
Product Details
| Product Name | Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM140 |
| Product Description | Rabbit monoclonal to Monomethylated Histone H3 Lysine 4, H3K4me1, Histone H3 (monomethyl K4) |
| Catalog No. | 31-1046-00 |
| Clone Name | RM140 |
| Product Data Sheet | Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM140 |
| Specificity | RM140 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3 |
| Immunogen | A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys4) |
| Application | Western Blot (WB) Chromatin IP (ChIP) Immunocytochemistry (ICC) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.2 µg/mL – 1 µg/mL; ChIP: 2 µg/mL- 10 µg/mL; ICC: 1 µg/mL - 2 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 0.5 µg/mL. |
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| References | Shah RN et al Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies. Mol Cell. (2018), 10.1016/j.molcel.2018.08.015. ICeChIP. Read more (PubMed: 30244833) |
Product Images
 | RM140 specifically reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3. |
 | Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM140 at 0.5 µg/mL, showed a band of histone H3 monomethylated at Lysine 4 (K4me1) in HeLa cells. |
 | ChIP performed on HeLa cells using H3K4me1 antibody (RM140, 5 µg). Real-time PCR was performed using primers specific to the gene indicated. |
 | Immunocytochemistry of HeLa cells treated with sodium butyrate, using Monomethyl-Histone H3 (Lys4) Rabbit mAb RM140 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
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