Anti-Mouse IgG2a Kappa Rabbit Monoclonal Antibody [Clone RM107]; 31-1003-00
![Anti-Mouse IgG2a Kappa Rabbit Monoclonal Antibody [Clone RM107]; 31-1003-00](/upload/image/products/RM107_WB-e1625768815947.png)
![Anti-Mouse IgG2a Kappa Rabbit Monoclonal Antibody [Clone RM107]; 31-1003-00](/upload/image/products/Anri-mouse-IgG2a-2.jpg)
![Anti-Mouse IgG2a Kappa Rabbit Monoclonal Antibody [Clone RM107]; 31-1003-00](/upload/image/products/Anri-mouse-IgG2a-3.jpg)
Key features and details
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Product Details
| Product Name | Anti-Mouse IgG2a Kappa Rabbit Monoclonal Antibody, Clone RM107 |
| Product Description | Rabbit monoclonal to Mouse IgG2a Kappa |
| Catalog No. | 31-1003-00 |
| Clone Name | RM107 |
| Product Data Sheet | Anti-Mouse IgG2a Kappa Light Chain Rabbit Monoclonal Antibody Data Sheet RM107 |
| Specificity | RM107 reacts to the Fab region of mouse IgG2aκ. No cross reactivity with mouse IgG2aλ, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The Fc region of RM107 has been engineered to eliminate Fc receptor binding. |
| Immunogen | Mouse IgG |
| Application | ELISA Western Blot (WB) (nonreduced only) |
| Species Reactivity | Mouse |
| Conjugate | None |
| Intended Use | Research Use Only |
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| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 0.005 µg/mL – 0.2 µg/mL; Western Blot (WB): 0.1 µg/mL - 0.5 µg/mL. |
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| Western blot of nonreduced (-) and reduced (+) mouse IgG2aκ (20 ng/lane), using 0.2 µg/mL of RevMAb clone RM107. This antibody only reacts to nonreduced Mouse IgG2aκ. |
 | ELISA of mouse immunoglobulins shows RM107 reacts to the Fab region of mouse IgG2aκ; no cross reactivity with IgG2aλ, IgG1, IgG3, IgM, IgA, IgE,human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM107 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | A titer ELISA of mouse IgG2aκ. The plate was coated with different amounts of mouse IgG2aκ. A serial dilution of RM107 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
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