Anti-Mouse IgG3 Rabbit Monoclonal Antibody [Clone RM218], 31-1094-00

Key features and details

  • Uniprot: P03987
  • Gene ID: 380795
  • Host Species: Rabbit
  • Species Reactivity: Mouse
  • Concentration: 1 mg/mL
  • Brand:
CAT.NO. : 31-1094-00
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Product Details
Product Details
Product Name Anti-Mouse IgG3 Rabbit Monoclonal Antibody, Clone RM218
Product Description Rabbit monoclonal to Mouse IgG3
Catalog No. 31-1094-00
Clone Name RM218
Product Data Sheet Anti-Mouse IgG3 Rabbit Monoclonal Antibody Data Sheet RM218
Specificity RM218 reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.
Immunogen Mouse IgG3
Application ELISA
Western Blot (WB)

Species Reactivity Mouse
Conjugate None
Intended Use Research Use Only
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size50 μg
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.005 μg/mL - 0.2 μg/mL;
Western Blot (WB):0.1 μg/mL - 0.5 μg/mL.
Keywords
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Product Images

ELISA of mouse immunoglobulins shows RM218 reacts to both mouse IgG3κ and IgG3λ; No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins.200 ng/mL, 50 ng/mL, or 10 ng/mL of RM218 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. A serial dilution of RM218 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit Ig Gas the secondary antibody.

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