Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody [Clone RM103]; 31-1007-00
![Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody [Clone RM103]; 31-1007-00](/upload/image/products/RM103_WB-e1625767764496.png)
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Key features and details
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Product Details
| Product Name | Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody, Clone RM103 |
| Product Description | Rabbit monoclonal to Mouse Ig Kappa Light Chain |
| Catalog No. | 31-1007-00 |
| Clone Name | RM103 |
| Product Data Sheet | Anti-Mouse Ig Kappa Rabbit Monoclonal Antibody Data Sheet RM103 |
| Specificity | This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. |
| Immunogen | Mouse IgG |
| Application | ELISA Western Blot (WB) (nonreduced) |
| Species Reactivity | Mouse |
| Conjugate | None |
| Intended Use | Research Use Only |
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| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 0.005 µg/mL - 0.2 µg/mL; Western Blot (WB): 0.1 µg/mL - 0.5 µg/mL. |
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| Western blot of nonreduced (-) and reduced (+) mouse IgG1κ and IgG1λ (20 ng/lane), using 0.2 µg/mL of RevMAb Clone RM103. This antibody reacts to nonreduced IgG1κ (~150 kDa), and slightly reacts to reduced κ light chain (~25 kDa). |
 | ELISA of mouse immunoglobulins shows RM103 reacts to the kappa light chain of mouse immuno-globulins. No cross reactivity with the lamda light chain, human IgG (κ+λ), rat IgG (κ+λ), or goat IgG (κ+λ). The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | A titer ELISA of mouse IgG1κ. The plate was coated with different amounts of mouse IgG1κ. A serial dilution of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
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