anti-MSH6 antibody [SQab1720]
![anti-MSH6 antibody [SQab1720]](/upload/image/products/ARG66198_WB_Sheet_Rev_HeLa_170825.jpg)
![anti-MSH6 antibody [SQab1720]](/upload/image/products/ARG66198_IHC-P_human_colon.jpg)
![anti-MSH6 antibody [SQab1720]](/upload/image/products/ARG66198_WB_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG66198
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Product Details
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab1720] recognizes MSH6 |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | FACS, ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab1720 |
| 同位型 | IgG |
| 靶点名称 | MSH6 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the N-terminus of MSH6. |
| 偶联标记 | Un-conjugated |
| 別名 | DNA mismatch repair protein Msh6; HNPCC5; p160; GTMBP; G/T mismatch-binding protein; HSAP; hMSH6; GTBP; MutS-alpha 160 kDa subunit |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MSH6 |
| 全名 | mutS homolog 6 |
| 背景介绍 | MSH6 is a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013] |
| 生物功能 | MSH6 is a component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30312 DNA Mismatch Repair System Antibody Duo (MSH2, MSH6) Related products: MSH6 antibodies; MSH6 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| 研究领域 | DNA Mismatch Repair System Study antibody |
| 预测分子量 | 153 kDa |
| 翻译后修饰 | The N-terminus is blocked. Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway. |
检测图片 (3)
ARG66198 anti-MSH6 antibody [SQab1720] WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG66198 anti-MSH6 antibody [SQab1720] at 1:500 dilution.
ARG66198 anti-MSH6 antibody [SQab1720] IHC-P image
Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human colon tissue stained with ARG66198 anti-MSH6 antibody [SQab1720] at 1:500 dilution.
Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
ARG66198 anti-MSH6 antibody [SQab1720] WB image
Western blot: 20 µg of HeLa, A431, SW480, Rat testis and 293HEK cell lysates stained with ARG66198 anti-MSH6 antibody [SQab1720] at 1:5000 dilution.
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