anti-mTOR antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG54727
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes mTOR |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | mTOR |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 2459-2488 of Human mTOR (NP_004949.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Mammalian target of rapamycin; RAFT1; Mechanistic target of rapamycin; Rapamycin target protein 1; FRAP1; FRAP2; Rapamycin and FKBP12 target 1; FK506-binding protein 12-rapamycin complex-associated protein 1; mTOR; RAPT1; FKBP12-rapamycin complex-associated protein; FRAP; Serine/threonine-protein kinase mTOR; EC 2.7.11.1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 纯化 | Protein A purified |
|---|---|
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P42345 Human Serine/threonine-protein kinase mTOR |
|---|---|
| 基因名称 | MTOR |
| 全名 | mechanistic target of rapamycin (serine/threonine kinase) |
| 背景介绍 | The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008] |
| 生物功能 | Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. [From Uniprot] |
| 细胞定位 | Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome Cytoplasm. Nucleus, PML body Note=Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB |
| 产品亮点 | Related products: mTOR antibodies; mTOR Duos / Panels; Related news: Baking soda restores circadian clock in tumor cells |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody |
| 预测分子量 | 289 kDa |
| 翻译后修饰 | Autophosphorylates when part of mTORC1 or mTORC2. Phosphorylation at Ser-1261, Ser-2159 and Thr-2164 promotes autophosphorylation. Phosphorylation in the kinase domain modulates the interactions of MTOR with RPTOR and PRAS40 and leads to increased intrinsic mTORC1 kinase activity. Phosphorylation at Thr-2173 in the ATP-binding region by AKT1 strongly reduces kinase activity. |
检测图片 (3)
ARG54727 anti-mTOR antibody WB image
Western blot: 30 µg of PC3 cell lysate stained with ARG54727 anti-mTOR antibody at 1:1000 dilution.
ARG54727 anti-mTOR antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG54727 anti-mTOR antibody. DAPI (blue) for nuclear staining.
ARG54727 anti-mTOR antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue stained with ARG54727 anti-mTOR antibody.
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