anti-MYL1 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes MYL1
  • 反应物种: Hu, Ms, Rat
  • 应用: FACS, ICC/IF, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: MYL1
  • 抗原物种: Human
  • 抗原: KLH-conjugated synthetic peptide between aa. 101-135 of Human MYL1.
  • Brand:
CAT.NO. : ARG42171
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes MYL1
反应物种Hu, Ms, Rat
应用FACS, ICC/IF, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称MYL1
抗原物种Human
抗原KLH-conjugated synthetic peptide between aa. 101-135 of Human MYL1.
偶联标记Un-conjugated
別名Myosin light chain A1/A2; MLC1F/MLC3F; MLC1F; MLC1/MLC3; Myosin light chain alkali 1/2; Myosin light chain 1/3, skeletal muscle isoform; MLC3F
应用说明
应用建议
应用推荐稀释比
FACS1:25
ICC/IF1:25
IHC-P1:25
WB1:2000
应用说明IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照Human heart
实际分子量~ 25 kDa
属性
形式Liquid
纯化Purification with Protein A and immunogen peptide.
缓冲液PBS and 0.09% (W/V) Sodium azide.
抗菌剂0.09% (W/V) Sodium azide
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 17901 Mouse MYL1

GeneID: 4632 Human MYL1

GeneID: 56781 Rat MYL1

基因名称MYL1
全名myosin, light chain 1, alkali; skeletal, fast
背景介绍Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]
生物功能Non-regulatory myosin light chain required for proper formation and/or maintenance of myofibers, and thus appropriate muscle function. [UniProt]
预测分子量21 kDa
翻译后修饰Isoform MLC3 is acetylated at position 2. [UniProt]
检测图片 (4)
  • ARG42171 anti-MYL1 antibody ICC/IF image

    Immunofluorescence: 4% Paraformaldehyde-fixed and 0.1% Triton X-100 permeabilized HeLa cells stained with ARG42171 anti-MYL1 antibody (green) at 1:25 dilution. DAPI (blue) for nuclear staining.

  • ARG42171 anti-MYL1 antibody IHC-P image

    Immunohistochemistry: Paraformaldehyde-fixed and paraffin-embedded Human heart tissue. Tissue was blocked with 3% BSA for 0.5 hour at room temperature. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Tissue section was stained with ARG42171 anti-MYL1 antibody at 1:25 dilution for 1 hour at 37°C.

  • ARG42171 anti-MYL1 antibody WB image

    Western blot: 20 µg of Human heart lysate stained with ARG42171 anti-MYL1 antibody at 1:2000 dilution.

  • ARG42171 anti-MYL1 antibody FACS image

    Flow Cytometry: HeLa cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. Cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by ARG42171 anti-MYL1 antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG1 (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

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