anti-Myosin Light Chain 2 antibody [3B2]

CAT.NO. : ARG56986

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Size:

50 μl

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [3B2] recognizes Myosin Light Chain 2
反应物种	Hu, Rat
应用	FACS, WB
宿主	Mouse
克隆	Monoclonal
克隆号	3B2
同位型	IgG2b, kappa
靶点名称	Myosin Light Chain 2
抗原物种	Human
抗原	Recombinant fragment around aa. 1-166 of Human Myosin Light Chain 2.
偶联标记	Un-conjugated
別名	MLC2; Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; CMH10; MLC-2; MLC-2v

应用说明

应用建议

应用	推荐稀释比
FACS	Assay-dependent
WB	1:1000 - 1:2000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 363925 Rat MYL2 GeneID: 4633 Human MYL2 Swiss-port # P08733 Rat Myosin regulatory light chain 2, ventricular/cardiac muscle isoform Swiss-port # P10916 Human Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
基因名称	MYL2
全名	myosin, light chain 2, regulatory, cardiac, slow
背景介绍	Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
预测分子量	19 kDa
翻译后修饰	N-terminus is methylated by METTL11A/NTM1. Phosphorylated by MYLK3 and MYLK2; promotes cardiac muscle contraction and function (By similarity). Dephosphorylated by PPP1CB complexed to PPP1R12B (By similarity). The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation); regulates cardiac torsion and workload distribution (By similarity).

检测图片 (2)

ARG56986 anti-Myosin Light Chain 2 antibody [3B2] FACS image
Flow Cytometry: A431 cell line stained with ARG56986 anti-Myosin Light Chain 2 antibody [3B2] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
ARG56986 anti-Myosin Light Chain 2 antibody [3B2] WB image
Western blot: 60 µg of Rat heart stained with ARG56986 anti-Myosin Light Chain 2 antibody [3B2] at 1:1000.

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