Anti-N6-Methyladenosine Rabbit Monoclonal Antibody [Clone RM362], 31-1248-00
![Anti-N6-Methyladenosine Rabbit Monoclonal Antibody [Clone RM362], 31-1248-00](/upload/image/products/RM362_DB_m6A.png)
![Anti-N6-Methyladenosine Rabbit Monoclonal Antibody [Clone RM362], 31-1248-00](/upload/image/products/Fig2_MeRIP_m6A_e.png)
Key features and details
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CAT.NO. : 31-1248-00
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Product Details
Product Details
| Product Name | Anti-N6-Methyladenosine (m6A) Rabbit Monoclonal Antibody, Clone RM362 |
| Product Description | Rabbit monoclonal to N6-Methyladenosine (m6A) |
| Catalog No. | 31-1248-00 |
| Clone Name | RM362 |
| Product Data Sheet | Anti-N6-Methyladenosine (m6A) Rabbit Monoclonal Antibody Data Sheet RM362 |
| Specificity | RM362 reacts to N6-Methyladenosine (m6A) in both RNA and DNA. No cross reactivity with non-methylated adenosine. |
| Immunogen | BSA-conjugated N6-Methyladenosine |
| Application | Dot Blot ELISA Methylated RNA Immunoprecipitation |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 50 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | DotBlot: 0.5 µg/mL – 2 µg/mL; ELISA: 0.1 µg/mL – 3 µg/mL; MeRIP: 0.2 µg/mL – 5 µg/mL |
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Product Images
 | Dot blot of Lambda DNA with and without m6A, using anti-m6A antibody, Clone RM362. The membrane was pre-spotted with 5, and 0.5 ng/dot of lambda DNA. m6A-free DNA was isolated from bacteriophage lambda grown in an E. coli host-deficient in adenine methylase (dam-). |
 | MeRIP was performed using M6A and control RNA from NEB (E1610). Antibodies (Anti-m6A RM362 and Anti-PEG RM105) were mixed for a short time, with Protein A beads before the addition of m6A labelled and non- labelled RNA (in equal amounts). Captured RNA was then washed, eluted and run through RT-PCR. Resulting cDNA was then analyzed using Realtime PCR with specific probes to m6A labelled and un-labelled sequences. |
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