anti-NCK2 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes NCK2
  • 反应物种: Hu, Ms, Rat
  • 应用: IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: NCK2
  • 抗原物种: Human
  • 抗原: Recombinant fusion protein corresponding to aa. 1-380 of Human NCK2 (NP_003572.2).
  • Brand:
CAT.NO. : ARG59810
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes NCK2
反应物种Hu, Ms, Rat
应用IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称NCK2
抗原物种Human
抗原Recombinant fusion protein corresponding to aa. 1-380 of Human NCK2 (NP_003572.2).
偶联标记Un-conjugated
別名Nck-2; NCKbeta; NCK adaptor protein 2; SH2/SH3 adaptor protein NCK-beta; GRB4; Growth factor receptor-bound protein 4; Cytoplasmic protein NCK2
应用说明
应用建议
应用推荐稀释比
IHC-P1:50 - 1:200
WB1:500 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照Rat brain, Mouse lung and 293T
实际分子量43 kDa
属性
形式Liquid
纯化Affinity purified.
缓冲液PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 17974 Mouse NCK2

GeneID: 8440 Human NCK2

Swiss-port # O43639 Human Cytoplasmic protein NCK2

Swiss-port # O55033 Mouse Cytoplasmic protein NCK2

基因名称NCK2
全名NCK adaptor protein 2
背景介绍This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
生物功能Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. [UniProt]
细胞定位Cytoplasm. Endoplasmic reticulum. [UniProt]
预测分子量43 kDa
翻译后修饰Phosphorylated. [UniProt]
检测图片 (2)
  • ARG59810 anti-NCK2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human prostate stained with ARG59810 anti-NCK2 antibody at 1:100 dilution.

  • ARG59810 anti-NCK2 antibody WB image

    Western blot: 25 µg of Rat brain, Mouse lung and 293T cell lysates stained with ARG59810 anti-NCK2 antibody at 1:1000 dilution.

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