anti-NCOR1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes NCOR1 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NCOR1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide around the N-terminus of Human NCOR1. |
| 偶联标记 | Un-conjugated |
| 別名 | N-CoR1; N-CoR; Nuclear receptor corepressor 1; TRAC1; PPP1R109; hN-CoR |
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Sodium citrate buffer (10mM, pH 6.0), Boiling bathing for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | Liquid (pH 7.3), 0.42% Potassium phosphate, 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 30% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | NCOR1 |
| 全名 | nuclear receptor corepressor 1 |
| 背景介绍 | This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.[provided by RefSeq, Jun 2010] |
| 生物功能 | Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. Participates in the transcriptional repressor activity produced by BCL6. [UniProt] |
| 预测分子量 | 270 kDa |
| 翻译后修饰 | Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation. |
ARG65813 anti-NCOR1 antibody ICC/IF image
Immunocytochemistry: Formalin-fixed HeLa cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 min and blocked with 3% BSA-PBS for 30 min at RT. Cells were stained with ARG65813 anti-NCOR1 antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at RT in the dark. DAPI was used to stain the cell nuclei (blue).
ARG65813 anti-NCOR1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer tissue section. Antigen retrieval: Sodium citrate buffer (10mM, pH 6.0), Boiling bathing for 20 min. The section was stained with ARG65813 anti-NCOR1 antibody at RT and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ARG65813 anti-NCOR1 antibody WB image
Western blot: K562 and HeLa whole cell lysates stained with ARG65813 anti-NCOR1 antibody.
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