anti-NEK7 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes NEK7
  • 反应物种: Hu, Ms, Rat
  • 预测物种: Cow, Dog
  • 应用: WB
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: NEK7
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG41722
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes NEK7
反应物种Hu, Ms, Rat
预测物种Cow, Dog
应用WB
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称NEK7
抗原物种Human
抗原Synthetic peptide around the C-terminus of Human NEK7. (NP_598001.1) (CINPDPEKRPDVT)
偶联标记Un-conjugated
別名EC 2.7.11.1; Never in mitosis A-related kinase 7; Serine/threonine-protein kinase Nek7; NimA-related protein kinase 7
应用说明
应用建议
应用推荐稀释比
WB0.1 - 0.3 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量~ 32 kDa
属性
形式Liquid
纯化Ammonium sulphate precipitation followed by affinity purification with immunogen.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 140609 Human NEK7

GeneID: 360850 Rat NEK7

GeneID: 59125 Mouse NEK7

基因名称NEK7
全名NIMA-related kinase 7
背景介绍NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002]
生物功能Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1. [UniProt]
细胞定位Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Present at centrosome throughout the cell cycle. Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody. [UniProt]
预测分子量35 kDa
翻译后修饰Phosphorylation at Ser-195 required for its activation. [UniProt]
检测图片 (2)
  • ARG41722 anti-NEK7 antibody WB image

    Western blot: 35 µg of Human lung lysate (in RIPA buffer) stained with ARG41722 anti-NEK7 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.

  • ARG41722 anti-NEK7 antibody WB image

    Western blot: 35 µg of Mouse lung, Mouse testis, Rat lung and Rat testis lysates (in RIPA buffer) stained with ARG41722 anti-NEK7 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.

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