anti-NEK7 antibody
CAT.NO. : ARG41722
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概述
产品描述 | Goat Polyclonal antibody recognizes NEK7 |
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反应物种 | Hu, Ms, Rat |
预测物种 | Cow, Dog |
应用 | WB |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | NEK7 |
抗原物种 | Human |
抗原 | Synthetic peptide around the C-terminus of Human NEK7. (NP_598001.1) (CINPDPEKRPDVT) |
偶联标记 | Un-conjugated |
別名 | EC 2.7.11.1; Never in mitosis A-related kinase 7; Serine/threonine-protein kinase Nek7; NimA-related protein kinase 7 |
应用说明
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
实际分子量 | ~ 32 kDa |
属性
形式 | Liquid |
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纯化 | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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基因名称 | NEK7 |
全名 | NIMA-related kinase 7 |
背景介绍 | NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002] |
生物功能 | Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1. [UniProt] |
细胞定位 | Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Present at centrosome throughout the cell cycle. Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody. [UniProt] |
预测分子量 | 35 kDa |
翻译后修饰 | Phosphorylation at Ser-195 required for its activation. [UniProt] |
检测图片 (2)
ARG41722 anti-NEK7 antibody WB image
Western blot: 35 µg of Human lung lysate (in RIPA buffer) stained with ARG41722 anti-NEK7 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
ARG41722 anti-NEK7 antibody WB image
Western blot: 35 µg of Mouse lung, Mouse testis, Rat lung and Rat testis lysates (in RIPA buffer) stained with ARG41722 anti-NEK7 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
