anti-Nephrin antibody [Y17-R]
![anti-Nephrin antibody [Y17-R]](/upload/image/products/ARG10579_WB_1_210_205.jpg)
![anti-Nephrin antibody [Y17-R]](/upload/image/products/ARG10579_ICC_1.jpg)
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Key features and details
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- 克隆:
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- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG10579
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Product Details
概述
| 产品描述 | Rabbit Monoclonal antibody [Y17-R] recognizes Nephrin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | Y17-R |
| 同位型 | IgG |
| 靶点名称 | Nephrin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the center of the fifth Ig-like domain of Human nephrin (between residues Cys465-Cys528) |
| 偶联标记 | Un-conjugated |
| 別名 | CNF; NPHN; Renal glomerulus-specific cell adhesion receptor; nephrin; Nephrin |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 10 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 10 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NPHS1 |
| 全名 | nephrosis 1, congenital, Finnish type (nephrin) |
| 背景介绍 | This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009] |
| 生物功能 | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity). [UniProt] |
| 预测分子量 | 135 kDa |
| 翻译后修饰 | Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. |
检测图片 (2)
ARG10579 anti-Nephrin antibody [Y17-R] ICC/IF image
Immunocytochemistry: HEK293 cells stained with ARG10579 anti-Nephrin antibody [Y17-R] at 1:700 dilution.
ARG10579 anti-Nephrin antibody [Y17-R] WB image
Western blot: 1) 50 ng, 2) 100 ng, and 3) 200 ng of recombinant Mouse nephrin (extracellular domain, Gln37 - Thr1049) stained with ARG10579 anti-Nephrin antibody [Y17-R].
参考文献
A glomerulus chip with spherically twisted cell-laden hollow fibers as glomerular capillary tufts
ICC/IF / Human
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