anti-Neurofilament NF-H antibody [RT97]
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- 靶点名称:
- 抗原物种:
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CAT.NO. : ARG55971
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [RT97] recognizes Neurofilament NF-H |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | RT97 |
| 同位型 | IgG1 |
| 靶点名称 | Neurofilament NF-H |
| 抗原物种 | Rat |
| 抗原 | The triton-X 100 insoluble protein fraction of Rat brain. |
| 偶联标记 | Un-conjugated |
| 別名 | Neurofilament heavy polypeptide; 200 kDa neurofilament protein; NF-H; Neurofilament triplet H protein; NFH |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Human brain |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Nefh |
| 全名 | neurofilament, heavy polypeptide |
| 背景介绍 | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008] |
| 生物功能 | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. [UniProt] |
| 细胞定位 | Cytoplasmic |
| 产品亮点 | Related products: Neurofilament NF H antibodies; Neurofilament NF H ELISA Kits; Neurofilament NF H Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Neuronal Development Marker |
| 研究领域 | Neuroscience antibody; Signaling Transduction antibody; Neurofilament antibody; Intermediate Neurofilament antibody |
| 预测分子量 | 112 kDa |
| 翻译后修饰 | There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. |
检测图片 (2)
ARG55971 anti-Neurofilament NF-H antibody [RT97] IHC-P image
Immunohistochemistry: Cerebellum stained with ARG55971 anti-Neurofilament NF-H antibody [RT97].
ARG55971 anti-Neurofilament NF-H antibody [RT97] WB image
Western blot: Human brain lysate stained with ARG55971 anti-Neurofilament NF-H antibody [RT97].
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