anti-Neurofilament NF-H phospho (KSP site) antibody [NAP4]

Key features and details

产品描述: Mouse Monoclonal antibody [NAP4] recognizes Neurofilament NF-H when KSP site was phosphorylated
反应物种: Ms, Rat, Cow, Pig
预测物种: Hu
应用: IHC-P, WB
特异性: NAP4 recognizes phosphorylated NF-H KSP sequences but not non-phosphorylated KSP sequences.
宿主: Mouse
克隆: Monoclonal
克隆号: NAP4
同位型: IgG1

Brand:

CAT.NO. : ARG52346

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Size:

50 μl

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*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [NAP4] recognizes Neurofilament NF-H when KSP site was phosphorylated
反应物种	Ms, Rat, Cow, Pig
预测物种	Hu
应用	IHC-P, WB
特异性	NAP4 recognizes phosphorylated NF-H KSP sequences but not non-phosphorylated KSP sequences.
宿主	Mouse
克隆	Monoclonal
克隆号	NAP4
同位型	IgG1
靶点名称	Neurofilament NF-H
抗原物种	Bovine
抗原	Purified bovine NF-H
偶联标记	Un-conjugated
別名	Neurofilament heavy polypeptide; 200 kDa neurofilament protein; NF-H; Neurofilament triplet H protein; NFH

应用说明

应用建议

应用	推荐稀释比
IHC-P	1:500 - 1:1000
WB	1:5000 - 1:10000

应用说明

Specific for the ~200k Neurofilament H protein. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Total IgG fraction
缓冲液	Total IgG fraction and 10 mM Sodium azide
抗菌剂	10 mM Sodium azide
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 100156492 Pig NEFH GeneID: 100625407 Pig NEFH GeneID: 380684 Mouse NEFH Swiss-port # P19246 Mouse Neurofilament heavy polypeptide
背景介绍	Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H . NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (2) and Alzheimer's disease .
研究领域	Neuroscience antibody; Signaling Transduction antibody; Neurofilament antibody; Intermediate Neurofilament antibody
预测分子量	112 kDa
翻译后修饰	There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.

检测图片 (3)

ARG52346 anti-Neurofilament NF-H antibody [NAP4] WB image
Western Blot: Rat cortex lysate showing specific immunolabeling of the ~200k NF-H protein stained with ARG52346 anti-Neurofilament NF-H antibody [NAP4].
ARG52346 anti-Neurofilament NF-H antibody [NAP4] IHC image
Immunohistochemistry: Human cerebellar cortex stained with ARG52346 anti-Neurofilament NF-H antibody [NAP4] showing labeling of NF-H (brown) in basket cell axons surrounding the large Purkinje neurons.
ARG52346 anti-Neurofilament NF-H antibody [NAP4] WB image
Western blot: Rat brain, Rat spinal cord, Mouse brain, Mouse spinal cord, Pig spinal cord and Cow spinal cord lysates stained with ARG52346 anti-Neurofilament NF-H antibody [NAP4] (green) at 1:10000 dilution.
Strong band at about 200-220 kDa corresponds to the major phosphorylated form of the NF-H subunit. A minor band at about 160 kDa is the non-phosphorylated NF-H. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations.