anti-Neurotensin Receptor 1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes Neurotensin Receptor 1 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Neurotensin Receptor 1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide encompassing a sequence within the center region of Human NTR1. |
| 偶联标记 | Un-conjugated |
| 別名 | NTR; NTR1; Neurotensin receptor type 1; NT-R-1; NTRH; High-affinity levocabastine-insensitive neurotensin receptor |
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 54 kDa |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 30% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | NTSR1 |
| 全名 | neurotensin receptor 1 (high affinity) |
| 背景介绍 | Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. [provided by RefSeq, Jul 2008] |
| 生物功能 | G-protein coupled receptor for the tridecapeptide neurotensin (NTS). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis. [UniProt] |
| 细胞定位 | Cell membrane; Multi-pass membrane protein. Membrane raft. Note=Palmitoylation is required for localization at CAV1-enriched membrane rafts. [UniProt] |
| 预测分子量 | 46 kDa |
| 翻译后修饰 | N-glycosylated. Palmitoylated; this is required for normal localization at membrane rafts and normal GNA11-mediated activation of down-stream signaling cascades. The palmitoylation level increases in response to neurotensin treatment. [UniProt] |
ARG66559 anti-Neurotensin Receptor 1 antibody ICC/IF image
Immunofluorescence: Formalin-fixed SK-N-SH cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66559 anti-Neurotensin Receptor 1 antibody (red) in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. DAPI was used to stain the cell nuclei (blue).
ARG66559 anti-Neurotensin Receptor 1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then stained with ARG66559 anti-Neurotensin Receptor 1 antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ARG66559 anti-Neurotensin Receptor 1 antibody WB image
Western blot: HeLa, MCF7 and Jurkat whole cell lysates stained with ARG66559 anti-Neurotensin Receptor 1 antibody.
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