anti-NFIA antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes NFIA
  • 反应物种: Hu, Ms, Rat
  • 应用: FACS, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: NFIA
  • 抗原物种: Human
  • 抗原: Synthetic peptide around aa. 180-224 of Human NFIA. (AYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGVFS)
  • Brand:
CAT.NO. : ARG23706
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes NFIA
反应物种Hu, Ms, Rat
应用FACS, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称NFIA
抗原物种Human
抗原Synthetic peptide around aa. 180-224 of Human NFIA. (AYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGVFS)
偶联标记Un-conjugated
別名Nuclear factor 1/A; Nuclear factor 1 A-type; TGGCA-binding protein; NF-I/A; NF1-A; CTF; CCAAT-box-binding transcription factor; NFI-L; Nuclear factor I/A; NFI-A
应用说明
应用建议
应用推荐稀释比
FACS1 - 3 µg/10^6 cells
IHC-P1 - 2 µg/ml
WB0.5 - 1 µg/ml
应用说明IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS, 0.025% Sodium azide and 2.5% BSA.
抗菌剂0.025% Sodium azide
稳定剂2.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 18027 Mouse NFIA

GeneID: 25492 Rat NFIA

GeneID: 4774 Human NFIA

基因名称NFIA
全名nuclear factor I/A
背景介绍This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
生物功能Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. [UniProt]
预测分子量56 kDa (unmodified); 60-70 kDa (phosphorylated)
检测图片 (6)
  • ARG23706 anti-NFIA antibody FACS image

    Flow Cytometry: Human K562 cells stained with ARG23706 anti-NFIA antibody at 1 µg/10^6 cells (blocked with Goat sera).

    Red: Cells alone
    Green: Isotype control
    Blue: Primary antibody.

  • ARG23706 anti-NFIA antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer tissue stained with ARG23706 anti-NFIA antibody at 1 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min.

  • ARG23706 anti-NFIA antibody WB image

    Western blot: 1) Jurkat and 2) COLO320 cell lysates stained with ARG23706 anti-NFIA antibody at 0.5 µg/ml dilution.

  • ARG23706 anti-NFIA antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse liver stained with ARG23706 anti-NFIA antibody at 1 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min.

  • ARG23706 anti-NFIA antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat liver stained with ARG23706 anti-NFIA antibody at 1 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min.

  • ARG23706 anti-NFIA antibody WB image

    Western blot: 1) HEPA1-6 and 2) SP2/0 cell lysates stained with ARG23706 anti-NFIA antibody at 0.5 µg/ml dilution.

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