anti-NME2 antibody [2E3]

CAT.NO. : ARG57502

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Size:

50 μl

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [2E3] recognizes NME2
反应物种	Hu
应用	ICC/IF, WB
宿主	Mouse
克隆	Monoclonal
克隆号	2E3
同位型	IgG1, kappa
靶点名称	NME2
抗原物种	Human
抗原	Recombinant Human NME2 (aa. 1-152) purified from E. coli.
偶联标记	Un-conjugated
別名	Nucleoside diphosphate kinase B; NDPK-B; NDP kinase B; NDKB; NDPKB; EC 2.7.13.3; PUF; Histidine protein kinase NDKB; NDK B; EC 2.7.4.6; NM23B; C-myc purine-binding transcription factor PUF; NM23-H2; nm23-H2

应用说明

应用建议

应用	推荐稀释比
ICC/IF	1:100
WB	1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 4831 Human NME2 Swiss-port # P22392 Human Nucleoside diphosphate kinase B
基因名称	NME2
全名	NME/NM23 nucleoside diphosphate kinase 2
背景介绍	Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010]
生物功能	Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically. Exhibits histidine protein kinase activity. [UniProt]
预测分子量	17 kDa

检测图片 (3)

ARG57502 anti-NME2 antibody [2E3] ICC/IF image
Immunoflorescense: HeLa cells stained with ARG57502 anti-NME2 antibody [2E3] at 1:100 dilution. The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ARG57502 anti-NME2 antibody [2E3] WB image
Western blot: 40 µg of HeLa, A549, MCF7 and LnCap cell lysates stained with ARG57502 anti-NME2 antibody [2E3] at 1:1000 dilution.
ARG57502 anti-NME2 antibody [2E3] WB image
Western blot: 5 µg of 293T and NME2 transfected 293T cell lysates stained with ARG57502 anti-NME2 antibody [2E3] at 1:1000 dilution.

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