anti-NOVA2 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes NOVA2
  • 反应物种: Hu, Ms, Rat
  • 应用: FACS, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: NOVA2
  • 抗原物种: Human
  • 抗原: Recombinant protein corresponding to M1-Q205 of Human NOVA2.
  • Brand:
CAT.NO. : ARG42975
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes NOVA2
反应物种Hu, Ms, Rat
应用FACS, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称NOVA2
抗原物种Human
抗原Recombinant protein corresponding to M1-Q205 of Human NOVA2.
偶联标记Un-conjugated
別名NOVA3; RNA-binding protein Nova-2; Astrocytic NOVA1-like RNA-binding protein; ANOVA; Neuro-oncological ventral antigen 2
应用说明
应用建议
应用推荐稀释比
FACS1:150 - 1:500
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
应用说明IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量49, 72 kDa
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
抗菌剂0.05% Sodium azide
稳定剂4% Trehalose
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 4858 Human NOVA2

Swiss-port # Q9UNW9 Human RNA-binding protein Nova-2

基因名称NOVA2
全名neuro-oncological ventral antigen 2
生物功能May regulate RNA splicing or metabolism in a specific subset of developing neurons (By similarity). Binds single strand RNA. [UniProt]
细胞定位Nucleus. [UniProt]
预测分子量49 kDa
检测图片 (7)
  • ARG42975 anti-NOVA2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human appendicitis tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42975 anti-NOVA2 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG42975 anti-NOVA2 antibody WB image

    Western blot: 50 µg of sample under reducing conditions. U2OS, HL-60, THP-1, Rat brain, Rat spleen, Rat lung and Mouse brain lysates stained with ARG42975 anti-NOVA2 antibody at 0.5 µg/ml dilution, overnight at 4°C.

  • ARG42975 anti-NOVA2 antibody FACS image

    Flow Cytometry: A549 cells were blocked with 10% normal goat serum and then stained with ARG42975 anti-NOVA2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

  • ARG42975 anti-NOVA2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42975 anti-NOVA2 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG42975 anti-NOVA2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42975 anti-NOVA2 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG42975 anti-NOVA2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat liver tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42975 anti-NOVA2 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG42975 anti-NOVA2 antibody FACS image

    Flow Cytometry: Hepa 1-6 cells were blocked with 10% normal goat serum and then stained with ARG42975 anti-NOVA2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

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