anti-Nrf 2 phospho (Ser40) antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
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- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG40667
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Nrf 2 phospho (Ser40) |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Nrf 2 |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide around Ser40 of Human Nrf 2. |
| 偶联标记 | Un-conjugated |
| 別名 | Nuclear factor, erythroid derived 2, like 2; NF-E2-related factor 2; Nuclear factor erythroid 2-related factor 2; HEBP1; NRF2; NFE2-related factor 2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q16236 Human Nuclear factor erythroid 2-related factor 2 |
|---|---|
| 基因名称 | NFE2L2 |
| 全名 | nuclear factor, erythroid 2-like 2 |
| 背景介绍 | This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015] |
| 生物功能 | Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. [UniProt] |
| 产品亮点 | Related products: Nrf 2 antibodies; Nrf 2 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Keap1-Nrf2-ARE antibody panel is launched |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; NRF-2 Nuclear Localization antibody |
| 预测分子量 | 68 kDa |
| 翻译后修饰 | Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. Acetylation at Lys-596 and Lys-599 increases nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence. Ubiquitinated by the KEAP1-CUL3-RBX1 E3 ubiquitin ligase complex and subject to proteasomal degradation. Ubiquitination is inhibited by sulforaphane. [UniProt] |
检测图片 (3)
ARG40667 anti-Nrf 2 phospho (Ser40) antibody ICC/IF image
Immunofluorescence: HepG2 cells stained with ARG40667 anti-Nrf 2 phospho (Ser40) antibody.
ARG40667 anti-Nrf 2 phospho (Ser40) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma stained with ARG40667 anti-Nrf 2 phospho (Ser40) antibody.
ARG40667 anti-Nrf 2 phospho (Ser40) antibody WB image
Western blot: HepG2 cell lysate stained with ARG40667 anti-Nrf 2 phospho (Ser40) antibody.
参考文献
Blockage of Nrf2 and autophagy by L-selenocystine induces selective death in Nrf2-addicted colorectal cancer cells through p62-Keap-1-Nrf2 axis
WB, IHC-P / Human
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