anti-NSE / Neuron Specific Enolase antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes NSE / Neuron Specific Enolase
  • 反应物种: Hu
  • 预测物种: Ms, Rat
  • 应用: ICC/IF, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: NSE / Neuron Specific Enolase
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG66864
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes NSE / Neuron Specific Enolase
反应物种Hu
预测物种Ms, Rat
应用ICC/IF, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称NSE / Neuron Specific Enolase
抗原物种Human
抗原Synthetic peptide corresponding to aa. 371-420 of Human NSE / Neuron Specific Enolase.
偶联标记Un-conjugated
別名Neural enolase; NSE; Enolase 2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; HEL-S-279; Neuron-specific enolase; EC 4.2.1.11
应用说明
应用建议
应用推荐稀释比
ICC/IF1:200 - 1:1000
IHC-P1:100 - 1:300
WB1:500 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照HepG2
实际分子量~ 48 kDa
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol and 0.5% BSA
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 2026 Human ENO2

Swiss-port # P09104 Human Gamma-enolase

基因名称ENO2
全名enolase 2 (gamma, neuronal)
背景介绍This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
生物功能Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). [UniProt]
细胞定位Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. [UniProt]
预测分子量47 kDa
检测图片 (4)
  • ARG66864 anti-NSE / Neuron Specific Enolase antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG66864 anti-NSE / Neuron Specific Enolase antibody (green) at 1:200 dilution, overnight at 4°C. DAPI (blue) for nuclear staining.

  • ARG66864 anti-NSE / Neuron Specific Enolase antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG66864 anti-NSE / Neuron Specific Enolase antibody. The picture on the right is blocked with the synthetic peptide.

  • ARG66864 anti-NSE / Neuron Specific Enolase antibody WB image

    Western blot: HepG2 cell lysate stained with ARG66864 anti-NSE / Neuron Specific Enolase antibody at 1:2000 dilution.

  • ARG66864 anti-NSE / Neuron Specific Enolase antibody ICC/IF image

    Immunofluorescence: A549 cells stained with ARG66864 anti-NSE / Neuron Specific Enolase antibody. The picture on the right is blocked with the synthetic peptide.

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