anti-NSFL1C antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG40146
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NSFL1C |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NSFL1C |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 214-248 of Human NSFL1C. |
| 偶联标记 | Un-conjugated |
| 別名 | p97 cofactor p47; dJ776F14.1; UBXD10; UBX domain-containing protein 2C; NSFL1 cofactor p47; UBX1; UBXN2C; P47 |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | 293 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NSFL1C |
| 全名 | NSFL1 (p97) cofactor (p47) |
| 背景介绍 | N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, May 2011] |
| 生物功能 | Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro). [UniProt] |
| 细胞定位 | Nucleus. Golgi apparatus, Golgi stack. Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks. Localizes to centrosome during mitotic prophase and metaphase. [UniProt] |
| 预测分子量 | 41 kDa |
| 翻译后修饰 | Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis (By similarity). [UniProt] |
检测图片 (2)
ARG40146 anti-NSFL1C antibody IHC-P image
Immunohistochemistry: Paraformaldehyde-fixed and paraffin-embedded Human testis was blocked with 3% BSA for 0.5 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Samples were stained with ARG40146 anti-NSFL1C antibody at 1:25 for 1 hour at 37°C.
ARG40146 anti-NSFL1C antibody WB image
Western blot: 20 µg of 293 cell lysate stained with ARG40146 anti-NSFL1C antibody at 1:2000 dilution.
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