anti-NSUN2 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal recognizes NSUN2
  • 反应物种: Hu, Ms, Rat
  • 应用: FACS, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: NSUN2
  • 抗原物种: Human
  • 抗原: Human NSUN2 recombinant protein (Position: H146-K654).
  • Brand:
CAT.NO. : ARG44128
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Product Details
概述
产品描述Rabbit Polyclonal recognizes NSUN2
反应物种Hu, Ms, Rat
应用FACS, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称NSUN2
抗原物种Human
抗原Human NSUN2 recombinant protein (Position: H146-K654).
偶联标记Un-conjugated
別名NSUN2; NOP2/Sun RNA Methyltransferase 2; TRM4; SAKI; RNA Cytosine C(5)-Methyltransferase NSUN2; Misu; Myc-Induced SUN-Domain-Containing Protein; Myc-Induced SUN Domain-Containing Protein; NOL1/NOP2/Sun Domain Family, Member 2; MRNA Cytosine C(5)-Methyltransferase; TRNA Cytosine C(5)-Methyltransferase; Substrate Of AIM1/Aurora Kinase B; NOP2/Sun Domain Family, Member 2; TRNA Methyltransferase 4 Homolog; FLJ20303; MRT5; Mental Retardation, Non-Syndromic, Autosomal Recessive, 5; TRNA Methyltransferase 4 Homolog (S. Cerevisiae); NOP2/Sun RNA Methyltransferase Family Member 2; TRNA (Cytosine(34)-C(5))-Methyltransferase; TRNA (Cytosine-5-)-Methyltransferase NSUN2; NOL1/NOP2/Sun Domain Family Member 2; 5-Methycytoisine Methyltransferase; EC 2.1.1.203; EC 2.1.1.29; EC 2.1.1.-; EC 2.1.1; HTrm4; MISU
应用说明
应用建议
应用推荐稀释比
FACS1 - 3 µg/10^6 cells
IHC-P1 - 2 µg/ml
WB0.25-0.5 µg/ml
应用说明The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
抗菌剂0.05% Sodium azide
稳定剂4% Trehalose
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 28114 Mouse NSUN2

GeneID: 54888 Human NSUN2

Swiss-port # Q08J23 Human tRNA (cytosine(34)-C(5))-methyltransferase

Swiss-port # Q1HFZ0 Mouse tRNA (cytosine(34)-C(5))-methyltransferase

基因名称NSUN2
全名NOP2/Sun RNA Methyltransferase 2
背景介绍This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
生物功能RNA cytosine C5-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs).
细胞定位Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus, Secreted
预测分子量86 kDa
翻译后修饰Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation
检测图片 (7)
  • ARG44128 anti-NSUN2 antibody IHC-P image

    Immunohistochemistry: Human acinic cell carcinoma stained with ARG44128 anti-NSUN2 antibody at 2 μg/ml dilution.

  • ARG44128 anti-NSUN2 antibody WB image

    Western blot: Hela and 293T stained with ARG44128 anti-NSUN2 antibody at 0.5 μg/ml dilution.

  • ARG44128 anti-NSUN2 antibody FACS image

    Flow Cytometry: 293Tstained with ARG44128 anti-NSUN2 antibody at 1 µg/10^6 cells dilution.

  • ARG44128 anti-NSUN2 antibody WB image

    Western blot: Rat brain stained with ARG44128 anti-NSUN2 antibody at 0.5 μg/ml dilution.

  • ARG44128 anti-NSUN2 antibody WB image

    Western blot: Mouse brain stained with ARG44128 anti-NSUN2 antibody at 0.5 μg/ml dilution.

  • ARG44128 anti-NSUN2 antibody IHC-P image

    Immunohistochemistry: Human colorectal adenocarcinoma stained with ARG44128 anti-NSUN2 antibody at 2 μg/ml dilution.

  • ARG44128 anti-NSUN2 antibody IHC-P image

    Immunohistochemistry: Human ovarian cancer stained with ARG44128 anti-NSUN2 antibody at 2 μg/ml dilution.

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