anti-O-GalNAc + O-GlcNAc antibody [9B9]
产品描述 | Mouse Monoclonal antibody [9B9] recognizes O-GalNAc + O-GlcNAc |
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反应物种 | Other |
应用 | ICC/IF, IHC-P, WB |
特异性 | Specific for β-O-Linked N-Acetylgalactosamine and β-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins. |
宿主 | Mouse |
克隆 | Monoclonal |
克隆号 | 9B9 |
同位型 | IgM |
靶点名称 | O-GalNAc + O-GlcNAc |
抗原物种 | Others |
抗原 | Synthetic β-O-Linked N-Acetylglucosamine conjugated to KLH. |
偶联标记 | Un-conjugated |
应用建议 |
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应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
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纯化 | PEG Purified. |
缓冲液 | PBS (pH 7.4), 0.9% Sodium azide and 50% Glycerol. |
抗菌剂 | 0.9% Sodium azide |
稳定剂 | 50% Glycerol |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] ICC/IF image
Immunofluorescence: 5% Formaldehyde fixed (5 min) HEK293 cells stained with ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] (green) at 1:50 dilution for 30 - 60 min at RT. (A) DAPI (blue) for nuclear staining. (B) Phalloidin Alex Fluor 633 (red) for F-Actin staining. (C) Primary antibody. (D) Composite.
ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse colon tissue stained with ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] (green) at 1:25 dilution for 1 hour at RT. (A) DAPI (blue) for nuclear staining. (B) Phalloidin Alex Fluor 633 (red) for F-Actin staining. (C) Primary antibody. (D) Composite.
ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] WB image
Western blot: 2.0 µg of GlcNAc-BSA, GalNAc-BSA, Galactose-BSA, and Glucose-BSA stained with ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] at 1:1000 for 2 hours at RT.
